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http://hdl.handle.net/2445/177123
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DC Field | Value | Language |
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dc.contributor.author | Pineda Riu, Marta | - |
dc.contributor.author | Fernández, Esperanza | - |
dc.contributor.author | Torrents Arenales, David | - |
dc.contributor.author | Estévez Povedano, Raúl | - |
dc.contributor.author | López Iglesias, Carmen | - |
dc.contributor.author | Camps Camprubí, Marta | - |
dc.contributor.author | Lloberas Cavero, Jorge | - |
dc.contributor.author | Zorzano Olarte, Antonio | - |
dc.contributor.author | Palacín Prieto, Manuel | - |
dc.date.accessioned | 2021-05-10T13:27:46Z | - |
dc.date.available | 2021-05-10T13:27:46Z | - |
dc.date.issued | 1999-07-09 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/2445/177123 | - |
dc.description.abstract | We have identified a new human cDNA, L-amino acid transporter-2 (LAT-2), that induces a system L transport activity with 4F2hc (the heavy chain of the surface antigen 4F2, also named CD98) in oocytes. Human LAT-2 is the fourth member of the family of amino acid transporters that are subunits of 4F2hc. The amino acid transport activity induced by the co-expression of 4F2hc and LAT-2 was sodium-independent and showed broad specificity for small and large zwitterionic amino acids, as well as bulky analogs (e.g. BCH (2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid)). This transport activity was highly trans-stimulated, suggesting an exchanger mechanism of transport. Expression of tagged N-myc-LAT-2 alone in oocytes did not induce amino acid transport, and the protein had an intracellular location. Co-expression of N-myc-LAT-2 and 4F2hc gave amino acid transport induction and expression of N-myc-LAT-2 at the plasma membrane of the oocytes. These data suggest that LAT-2 is an additional member of the family of 4F2 light chain subunits, which associates with 4F2hc to express a system L transport activity with broad specificity for zwitterionic amino acids. Human LAT-2 mRNA is expressed in kidney >>> placenta >> brain, liver > spleen, skeletal muscle, heart, small intestine, and lung. Human LAT-2 gene localizes at chromosome 14q11.2-13 (13 cR or approximately 286 kb from marker D14S1349). The high expression of LAT-2 mRNA in epithelial cells of proximal tubules, the basolateral location of 4F2hc in these cells, and the amino acid transport activity of LAT-2 suggest that this transporter contributes to the renal reabsorption of neutral amino acids in the basolateral domain of epithelial proximal tubule cells. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1074/jbc.274.28.19738 | - |
dc.relation.ispartof | Journal of Biological Chemistry, 1999, vol. 274, num. 28, p. 19738-19744 | - |
dc.relation.uri | https://doi.org/10.1074/jbc.274.28.19738 | - |
dc.rights | (c) American Society for Biochemistry and Molecular Biology, 1999 | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Antígens CD | - |
dc.subject.classification | Proteïnes portadores | - |
dc.subject.classification | Proteïnes de membrana | - |
dc.subject.other | CD antigens | - |
dc.subject.other | Carrier proteins | - |
dc.subject.other | Membrane proteins | - |
dc.title | Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 147166 | - |
dc.date.updated | 2021-05-10T13:27:46Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 10391915 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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File | Description | Size | Format | |
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147166.pdf | 1.12 MB | Adobe PDF | View/Open |
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