Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/177357
Title: | Study of the protein complex, pore diameter, and pore-forming activity of the Borrelia burgdorferi P13 porin |
Author: | Bárcena Uribarri, Iván Thein, Marcus Barbot, Mariam Sans Serramitjana, Eulàlia Bonde, Mari Mentele, Reinhard Lottspeich, Friedrich Bergström, Sven Benz, Roland |
Keywords: | Bacteris Proteïnes Química Metabolisme Bacteria Proteins Chemistry Metabolism |
Issue Date: | 4-Jul-2014 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | P13 is one of the major outer membrane proteins of Borrelia burgdorferi. Previous studies described P13 as a porin. In the present study some structure and function aspects of P13 were studied. P13 showed according to lipid bilayer studies a channel-forming activity of 0.6 nanosiemens in 1 m KCl. Single channel and selectivity measurements demonstrated that P13 had no preference for either cations or anions and showed no voltage-gating up to ±100 mV. Blue native polyacrylamide gel electrophoresis was used to isolate and characterize the P13 protein complex in its native state. The complex had a high molecular mass of about 300 kDa and was only composed of P13 monomers. The channel size was investigated using non-electrolytes revealing an apparent diameter of about 1.4 nm with a 400-Da molecular mass cut-off. Multichannel titrations with different substrates reinforced the idea that P13 forms a general diffusion channel. The identity of P13 within the complex was confirmed by second dimension SDS-PAGE, Western blotting, mass spectrometry, and the use of a p13 deletion mutant strain. The results suggested that P13 is the protein responsible for the 0.6-nanosiemens pore-forming activity in the outer membrane of B. burgdorferi. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M113.539528 |
It is part of: | Journal of Biological Chemistry, 2014, vol. 289, num. 27, p. 18614-18624 |
URI: | http://hdl.handle.net/2445/177357 |
Related resource: | https://doi.org/10.1074/jbc.M113.539528 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
660906.pdf | 963.99 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.