Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/177363
Title: | MicroRNA-322 (miR-322) and its target protein Tob2 modulate Osterix (Osx) mRNA stability |
Author: | Gamez Molina, Beatriz Rodríguez Carballo, Edgardo Bartrons Bach, Ramon Rosa López, José Luis Ventura Pujol, Francesc |
Keywords: | Cicle cel·lular Expressió gènica Citologia Factors de transcripció Cell cycle Gene expression Cytology Transcription factors |
Issue Date: | 17-May-2013 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | Osteogenesis depends on a coordinated network of signals and transcription factors such as Runx2 and Osterix. Recent evidence indicates that microRNAs (miRNAs) act as important post-transcriptional regulators in a large number of processes, including osteoblast differentiation. In this study, we performed miRNA expression profiling and identified miR-322, a BMP-2-down-regulated miRNA, as a regulator of osteoblast differentiation. We report miR-322 gain- and loss-of-function experiments in C2C12 and MC3T3-E1 cells and primary cultures of murine bone marrow-derived mesenchymal stem cells. We demonstrate that overexpression of miR-322 enhances BMP-2 response, increasing the expression of Osx and other osteogenic genes. Furthermore, we identify Tob2 as a target of miR-322, and we characterize the specific Tob2 3′-UTR sequence bound by miR-322 by reporter assays. We demonstrate that Tob2 is a negative regulator of osteogenesis that binds and mediates degradation of Osx mRNA. Our results demonstrate a new molecular mechanism controlling osteogenesis through the specific miR-322/Tob2 regulation of specific target mRNAs. This regulatory circuit provides a clear example of a complex miRNA-transcription factor network for fine-tuning the osteoblast differentiation program. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M112.432104 |
It is part of: | Journal of Biological Chemistry, 2013, vol. 288, num. 20, p. 14264-14275 |
URI: | http://hdl.handle.net/2445/177363 |
Related resource: | https://doi.org/10.1074/jbc.M112.432104 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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622680.pdf | 2.54 MB | Adobe PDF | View/Open |
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