Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/177546
Title: | JunB is involved in the inhibition of myogenic differentiation by bone morphogenetic protein-2 |
Author: | Chalaux, Elisabet López Rovira, Teresa Rosa López, José Luis Bartrons Bach, Ramon Ventura Pujol, Francesc |
Keywords: | Ossos Proteïnes Músculs Factors de creixement Diferenciació cel·lular Bones Proteins Muscles Growth factors Cell diferentiation |
Issue Date: | 2-Jan-1998 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | Bone morphogenetic proteins (BMPs) constitute a family of multifunctional growth and differentiation factors structurally related to transforming growth factor-beta. BMPs were first identified by their osteoinductive effects, inducing ectopic bone formation when implanted in skeletal muscle, and have an important role as regulators of skeletal development in vivo. In vitro, BMP-2 is able to transdifferentiate myogenic C2C12 cells into the osteoblastic phenotype. In this report, we show that the osteoinductive effects of BMP-2 in C2C12 cells are mediated by bone morphogenetic protein receptor type-IA in combination with both activin receptor type II and bone morphogenetic protein receptor type II. We also analyzed the expression levels of nuclear protooncogenes to understand early transcriptional events induced by BMP-2. We show that junB is an immediate early gene induced by BMP-2 and transforming growth factor-beta. BMP-2 induces transcriptional activation of JunB expression as early as 30 min after ligand addition, reaching maximal levels after 90 min. Increase of JunB mRNA correlates with a higher AP-1 binding activity. Furthermore, ectopic overexpression of JunB is sufficient to inhibit expression of myoblast differentiation markers in C2C12 cells. These data, taken together, show the involvement of JunB in the early steps of inhibition of myogenic differentiation induced by transforming growth factor-beta family members. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.273.1.537 |
It is part of: | Journal of Biological Chemistry, 1998, vol. 273, num. 1, p. 537-543 |
URI: | http://hdl.handle.net/2445/177546 |
Related resource: | https://doi.org/10.1074/jbc.273.1.537 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
157358.pdf | 296.04 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.