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Title: Interaction of Transforming growth factor-β receptor I with farnesyl-protein transferase-α in yeast and mammalian cells
Author: Ventura Pujol, Francesc
Liu, Fang
Doody, Jacqueline
Massagué i Solé, Joan, 1953-
Keywords: Proteïnes quinases
Factors de creixement
Protein kinases
Growth factors
Issue Date: 14-Jun-1996
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: Transforming growth factor beta (TGF-beta) signals through two transmembrane serine/threonine kinases, known as TbetaR-I and TbetaR-II. Several lines of evidence suggest that TbetaR-II acts as a primary receptor, binding TGF-beta and phosphorylating TbetaR-I whose kinase activity then propagates the signal to unknown substrates. We report an interaction between TbetaR-I and the farnesyl-protein transferase-alpha subunit (FT-alpha) both in a yeast two-hybrid system and in mammalian cells. These findings raise the possibility that TGF-beta might regulate cellular functions by altering the ability of FT-alpha to catalyze isoprenylation of targets such as G proteins, lamins, or cytoskeletal components. However, we provide evidence that TGF-beta action does not alter the overall protein isoprenyl transferase activity in Mv1Lu mink lung epithelial cells. In fact, the beta subunits of farnesyl transferase and geranylgeranyl transferase, which are necessary for the activity of FT-alpha, prevent the association of FT-alpha with TbetaR-I. Furthermore, farnesyl transferase activity is shown to be dispensable for TGF-beta signaling of growth inhibitory and transcriptional responses in these cells. These results suggest that the interaction between TbetaR-I and FT-alpha does not affect the known functions of these two proteins.
Note: Reproducció del document publicat a:
It is part of: Journal of Biological Chemistry, 1996, vol. 271, num. 24, p. 13931-13934
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ISSN: 0021-9258
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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