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http://hdl.handle.net/2445/177612
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DC Field | Value | Language |
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dc.contributor.author | Soler Prat, Concepció | - |
dc.contributor.author | Felipe Campo, Antonio | - |
dc.contributor.author | Mata, João F. | - |
dc.contributor.author | Casado, Javier (Casado Merediz) | - |
dc.contributor.author | Celada Cotarelo, Antonio | - |
dc.contributor.author | Pastor Anglada, Marçal | - |
dc.date.accessioned | 2021-05-25T16:08:13Z | - |
dc.date.available | 2021-05-25T16:08:13Z | - |
dc.date.issued | 1998-10-09 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/2445/177612 | - |
dc.description.abstract | Nucleoside transport systems and their regulation in human B-lymphocytes have been characterized using the cell lines Raji and Bare lymphoma syndrome-1 (BLS-1) as experimental models. These cells express at least three different nucleoside transport systems as follows: a nitrobenzylthioinosine-sensitive equilibrative transport system of the es-type, which appears to be associated with hENT1 expression, and two Na+-dependent transport systems that may correspond to N1 and to the recently characterized N5-type, which is nitrobenzylthioinosine-sensitive and guanosine-preferring. B cell activators such as phorbol 12-myristate 13-acetate and lipopolysaccharide (LPS) up-regulate both concentrative transport systems but down-regulate the equilibrative es-type transporter, which correlates with lower hENT1 mRNA levels. These effects are dependent on protein kinase C activity. Phorbol 12-myristate 13-acetate and LPS also induce an increase in tumor necrosis factor-alpha (TNF-alpha) mRNA levels, which suggest that this cytokine may mediate some of the effects triggered by these agents, since addition of TNF-alpha alone can increase N1 and N5 transport activities by a mechanism that also depends on protein kinase C activation. Interestingly, TNF-alpha down-regulates es activity, but this effect cannot be abolished by inhibiting protein kinase C. This study reveals differential regulation of nucleoside transport systems following activation of human B-lymphocyte cell lines by agents of physiological relevance such as TNF-alpha and LPS. Moreover, it indicates that the recently characterized N5 transport system can also be regulated following B cell activation, which may be relevant to lymphocyte physiology and to the treatment of lymphocyte malignancies. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1074/jbc.273.41.26939 | - |
dc.relation.ispartof | Journal of Biological Chemistry, 1998, vol. 273, num. 41, p. 26939-26945 | - |
dc.relation.uri | https://doi.org/10.1074/jbc.273.41.26939 | - |
dc.rights | (c) American Society for Biochemistry and Molecular Biology, 1998 | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Limfòcits | - |
dc.subject.classification | Nucleòsids | - |
dc.subject.classification | Tumors | - |
dc.subject.classification | Farmacologia | - |
dc.subject.other | Lymphocytes | - |
dc.subject.other | Nucleosides | - |
dc.subject.other | Tumors | - |
dc.subject.other | Pharmacology | - |
dc.title | Regulation of nucleoside transport regulation by lipopolysaccharide, phorbol esters and TNF in human B lymphocytes | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 132353 | - |
dc.date.updated | 2021-05-25T16:08:13Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 9756942 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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File | Description | Size | Format | |
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132353.pdf | 407.8 kB | Adobe PDF | View/Open |
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