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http://hdl.handle.net/2445/177645
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DC Field | Value | Language |
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dc.contributor.author | Binz, Thomas | - |
dc.contributor.author | Blasi Cabús, Joan | - |
dc.contributor.author | Yamasaki, Shinji | - |
dc.contributor.author | Baumeister, Anja | - |
dc.contributor.author | Link, Egenhard | - |
dc.contributor.author | Südhof, Thomas C. | - |
dc.contributor.author | Jahn, Reinhard | - |
dc.contributor.author | Niemann, Heiner | - |
dc.date.accessioned | 2021-05-26T14:56:03Z | - |
dc.date.available | 2021-05-26T14:56:03Z | - |
dc.date.issued | 1994-01-21 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/2445/177645 | - |
dc.description.abstract | Clostridial neurotoxins, tetanus toxin (TeTx) and the seven related but serologically distinct botulinal neurotoxins (BoNT/A to BoNT/G), are potent inhibitors of synaptic vesicle exocytosis in nerve endings. Recently it was reported that the light chains of clostridial neurotoxins act as zinc-dependent metalloproteases which specifically cleave synaptic target proteins such as synaptobrevin/VAMPs, HPC-1/syntaxin (BoNT/C1), and SNAP-25 (BoNT/A). We show here that BoNT/E, like BoNT/A, cleaves SNAP-25, as generated by in vitro translation or by expression in Escherichia coli. BoNT/E cleaves the Arg180-Ile181 bond. This site is different from that of BoNT/A, which cleaves SNAP-25 between the amino acid residues Gln197 and Arg198. These findings further support the view that clostridial neurotoxins have evolved from an ancestral protease recognizing the exocytotic fusion machinery of synaptic vesicles whereby individual toxins target different members of the membrane fusion complex. | - |
dc.format.extent | 4 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isformatof | Reproducció del document publicat a: https://www.jbc.org/issue/S0021-9258(17)X4038-6 | - |
dc.relation.ispartof | Journal of Biological Chemistry, 1994, vol. 269, num. 3, p. 1617-1620 | - |
dc.rights | (c) American Society for Biochemistry and Molecular Biology, 1994 | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Toxina botulínica | - |
dc.subject.classification | Proteïnes de membrana | - |
dc.subject.classification | Teixit nerviós | - |
dc.subject.other | Botulinum toxin | - |
dc.subject.other | Membrane proteins | - |
dc.subject.other | Nerve tissue | - |
dc.title | Proteolysis of SNAP-25 by types E and A botulinal neurotoxins | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 092430 | - |
dc.date.updated | 2021-05-26T14:56:03Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 8294407 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) |
Files in This Item:
File | Description | Size | Format | |
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092430.pdf | 3.75 MB | Adobe PDF | View/Open |
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