Please use this identifier to cite or link to this item:
Title: Rabbit neurospheres as a novel in vitro tool for studying neurodevelopmental effects induced by intrauterine growth restriction
Author: Barenys Espadaler, Marta
Illa Armengol, Míriam
Hofrichter, Maxi
Loreiro, Carla
Pla, Laura
Klose, Jördis
Kühne, Britta Anna
Gómez Catalán, Jesús
Braun, Jan Matthias
Crispi Brillas, Fàtima
Gratacós Solsona, Eduard
Fritsche, Ellen
Keywords: Neurobiologia del desenvolupament
Creixement fetal
Developmental neurobiology
Fetal growth
Issue Date: 9-Oct-2020
Publisher: John Wiley & Sons
Abstract: The aim of this study was to develop a rabbit neurosphere culture to characterize differences in basic processes of neurogenesis induced by intrauterine growth restriction (IUGR). A novel in vitro neurosphere culture has been established using fresh or frozen neural progenitor cells from newborn (PND0) rabbit brains. After surgical IUGR induction in pregnant rabbits and cesarean section 5 days later, neural progenitor cells from both control and IUGR groups were isolated and directly cultured or frozen at −80°C. These neural progenitor cells spontaneously formed neurospheres after 7 days in culture. The ability of control and IUGR neurospheres to migrate, proliferate, differentiate to neurons, astrocytes, or oligodendrocytes was compared and the possibility to modulate their responses was tested by exposure to several positive and negative controls. Neurospheres obtained from IUGR brains have a significant impairment in oligodendrocyte differentiation, whereas no significant differences are observed in other basic processes of neurogenesis. This impairment can be reverted by in vitro exposure of IUGR neurospheres to thyroid hormone, which is known to play an essential role in white matter maturation in vivo. Our new rabbit neurosphere model and the results of this study open the possibility to test several substances in vitro as neuroprotective candidates against IUGR induced neurodevelopmental damage while decreasing the number of animals and resources and allowing a more mechanistic approach at a cellular functional level.
Note: Reproducció del document publicat a:
It is part of: Stem Cells Translational Medicine, 2020, vol. 10, p. 209-221
Related resource:
ISSN: 2157-6564
Appears in Collections:Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)
Articles publicats en revistes (BCNatal Fetal Medicine Research Center)

Files in This Item:
File Description SizeFormat 
703776.pdf1.62 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons