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http://hdl.handle.net/2445/178754
Title: | Analysis of ECs and related compounds in plasma: artifactual isomerization and ex vivo enzymatic generation of 2-MGs |
Author: | Pastor, Antoni Farré Albaladejo, Magí Fitó Colomer, Montserrat Fernández Aranda, Fernando Torre Fornell, Rafael de la |
Keywords: | Àcid araquidònic Anàlisi de sang Lipases Arachidonic acid Analysis of blood Lipase |
Issue Date: | 1-May-2014 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | The analysis of peripheral endocannabinoids (ECs) is a good biomarker of the EC system. Their concentrations, from clinical studies, strongly depend on sample collection and time processing conditions taking place in clinical and laboratory settings. The analysis of 2-monoacylglycerols (MGs) (i.e., 2-arachidonoylglycerol or 2-oleoylglycerol) is a particularly challenging issue because of their ex vivo formation and chemical isomerization that occur after blood sample collection. We provide evidence that their ex vivo formation can be minimized by adding Orlistat, an enzymatic lipase inhibitor, to plasma. Taking into consideration the low cost of Orlistat, we recommend its addition to plasma collecting tubes while maintaining sample cold chain until storage. We have validated a method for the determination of the EC profile of a range of MGs and N-acylethanolamides in plasma that preserves the original isomer ratio of MGs. Nevertheless, the chemical isomerization of 2-MGs can only be avoided by an immediate processing and analysis of samples due to their instability during conservation. We believe that this new methodology can aid in the harmonization of the measurement of ECs and related compounds in clinical samples. |
Note: | Reproducció del document publicat a: https://doi.org/10.1194/jlr.D043794 |
It is part of: | Journal of Lipid Research, 2014, vol. 55, num. 5, p. 966-977 |
URI: | http://hdl.handle.net/2445/178754 |
Related resource: | https://doi.org/10.1194/jlr.D043794 |
ISSN: | 0022-2275 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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647015.pdf | 1.06 MB | Adobe PDF | View/Open |
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