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Title: | Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids |
Author: | Adelfinskaya, O. Terrazas Martínez, Montserrat Froeyen, M. Marlière, P. Nauwelaerts, K. Herdewijn, P. |
Keywords: | ADN Aminoàcids Polímers DNA Amino acids Polymers |
Issue Date: | 25-Jul-2007 |
Publisher: | Oxford University Press |
Abstract: | Some selected amino acids, in particular L-aspartic acid (L-Asp) and L-histidine (L-His), can function as leaving group during polymerase-catalyzed incorporation of deoxyadenosine monophosphate (dAMP) in DNA. Although L-Asp-dAMP and L-His-dAMP bind, most probably, in a different way in the active site of the enzyme, aspartic acid and histidine can be considered as mimics of the pyrophosphate moiety of deoxyadenosine triphosphate. L-Aspartic acid is more efficient than D-aspartic acid as leaving group. Such P-N conjugates of amino acids and deoxynucleotides provide a novel experimental ground for diversifying nucleic acid metabolism in the field of synthetic biology. |
Note: | Reproducció del document publicat a: https://doi.org/10.1093/nar/gkm498 |
It is part of: | Nucleic Acids Research, 2007, vol. 35, num. 15, p. 5060-5072 |
URI: | http://hdl.handle.net/2445/179405 |
Related resource: | https://doi.org/10.1093/nar/gkm498 |
ISSN: | 0305-1048 |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) |
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