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Title: Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
Author: Vengut Climent, Empar
Terrazas Martínez, Montserrat
Lucas, Ricardo
Arévalo Ruiz, Matilde
Eritja i Casadellà, Ramon
Morales, Juan Carlos
Keywords: RNA
Química orgànica
Organic chemistry
Issue Date: 15-Jul-2013
Publisher: Elsevier Ltd
Abstract: Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation.
Note: Versió postprint del document publicat a:
It is part of: Bioorganic & Medicinal Chemistry Letters, 2013, vol. 23, num. 14, p. 4048-4051
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ISSN: 0960-894X
Appears in Collections:Articles publicats en revistes (Química Inorgànica i Orgànica)

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