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http://hdl.handle.net/2445/179846
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DC Field | Value | Language |
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dc.contributor.author | Monguió Tortajada, Marta | - |
dc.contributor.author | Prat Vidal, Cristina | - |
dc.contributor.author | Moron Font, Miriam | - |
dc.contributor.author | Clos Sansalvador, Marta | - |
dc.contributor.author | Calle, Alexandra | - |
dc.contributor.author | Gastelurrutia, Paloma | - |
dc.contributor.author | Cserkoova, Adriana | - |
dc.contributor.author | Morancho, Anna | - |
dc.contributor.author | Ramírez, Miguel Ángel | - |
dc.contributor.author | Rosell, Anna | - |
dc.contributor.author | Bayés Genís, Antoni | - |
dc.contributor.author | Gálvez Montón, Carolina | - |
dc.contributor.author | Borràs, Francesc E. | - |
dc.contributor.author | Roura, Santiago | - |
dc.date.accessioned | 2021-09-03T06:44:40Z | - |
dc.date.available | 2021-09-03T06:44:40Z | - |
dc.date.issued | 2021-07-01 | - |
dc.identifier.uri | http://hdl.handle.net/2445/179846 | - |
dc.description.abstract | The administration of extracellular vesicles (EV) from mesenchymal stromal cells (MSC) is a promising cell-free nanotherapy for tissue repair after myocardial infarction (MI). However, the optimal EV delivery strategy remains undetermined. Here, we designed a novel MSC-EV delivery, using 3D scaffolds engineered from decellularised cardiac tissue as a cell-free product for cardiac repair. EV from porcine cardiac adipose tissue-derived MSC (cATMSC) were purified by size exclusion chromatography (SEC), functionally analysed and loaded to scaffolds. cATMSC-EV markedly reduced polyclonal proliferation and pro-inflammatory cytokines production (IFNγ, TNFα, IL12p40) of allogeneic PBMC. Moreover, cATMSC-EV recruited outgrowth endothelial cells (OEC) and allogeneic MSC, and promoted angiogenesis. Fluorescently labelled cATMSC-EV were mixed with peptide hydrogel, and were successfully retained in decellularised scaffolds. Then, cATMSC-EV-embedded pericardial scaffolds were administered in vivo over the ischemic myocardium in a pig model of MI. Six days from implantation, the engineered scaffold efficiently integrated into the post-infarcted myocardium. cATMSC-EV were detected within the construct and MI core, and promoted an increase in vascular density and reduction in macrophage and T cell infiltration within the damaged myocardium. The confined administration of multifunctional MSC-EV within an engineered pericardial scaffold ensures local EV dosage and release, and generates a vascularised bioactive niche for cell recruitment, engraftment and modulation of short-term post-ischemic inflammation. | - |
dc.format.extent | 14 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.bioactmat.2021.02.026 | - |
dc.relation.ispartof | Bioactive Materials, 2021, vol. 6, num. 10, p. 3314-3327 | - |
dc.relation.uri | https://doi.org/10.1016/j.bioactmat.2021.02.026 | - |
dc.rights | cc by-nc-nd (c) Monguió Tortajada, Marta et al., 2021 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Enginyeria de teixits | - |
dc.subject.classification | Infart de miocardi | - |
dc.subject.other | Tissue engineering | - |
dc.subject.other | Myocardial infarction | - |
dc.title | Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2021-07-29T08:15:23Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 33778207 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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1-s2.0-S2452199X21000840-main.pdf | 12.54 MB | Adobe PDF | View/Open |
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