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http://hdl.handle.net/2445/179962
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DC Field | Value | Language |
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dc.contributor.author | Hernández López, Rubí | - |
dc.contributor.author | Hermosillo, Luis | - |
dc.contributor.author | León Maldonado, Leith | - |
dc.contributor.author | Velázquez Cruz, Rafael | - |
dc.contributor.author | Torres Ibarra, Leticia | - |
dc.contributor.author | Lazcano Ponce, Eduardo | - |
dc.contributor.author | Lorincz, Attila Tibor | - |
dc.contributor.author | Wheeler, Cosette M. | - |
dc.contributor.author | Bosch José, Francesc Xavier, 1947- | - |
dc.contributor.author | Cuzick, Jack | - |
dc.contributor.author | Rivera Paredez, Berenice | - |
dc.contributor.author | Nedjai, Belinda | - |
dc.contributor.author | Salmerón, Jorge | - |
dc.date.accessioned | 2021-09-13T10:35:00Z | - |
dc.date.available | 2021-09-13T10:35:00Z | - |
dc.date.issued | 2021-07-21 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/179962 | - |
dc.description.abstract | Introduction: Urine self-sampling for human papillomavirus (HPV)-based cervical cancer screening is a non-invasive method that offers several logistical advantages and high acceptability, reducing barriers related to low screening coverage. This study developed and evaluated the performance of a low-cost urine self-sampling method for HPV-testing and explored the acceptability and feasibility of potential implementation of this alternative in routine screening. Methods: A series of sequential laboratory assays examined the impact of several pre-analytical conditions for obtaining DNA from urine and subsequent HPV detection. Initially, we assessed the effect of ethylaminediaminetetraacetic acid (EDTA) as a DNA preservative examining several variables including EDTA concentration, specimen storage temperature, time between urine collection and DNA extraction, and first-morning micturition versus convenience sample collection. We further evaluated the agreement of HPV-testing between urine and clinician-collected cervical samples among 95 women. Finally, we explored the costs of self-sampling supplies as well as the acceptability and feasibility of urine self-sampling among women and healthcare workers. Results: Our results revealed higher DNA concentrations were obtained when using a 40mM EDTA solution, storing specimens at 25°C and extracting DNA within 72 hrs. of urine collection, regardless of using first-morning micturition or a convenience sampling. We observed good agreement (Kappa = 0.72) between urine and clinician-collected cervical samples for HPV detection. Furthermore, urine self-sampling was an affordable method (USD 1.10), well accepted among cervical cancer screening users, healthcare workers, and decision-makers. Conclusion: These results suggest urine self-sampling is feasible and appropriate alternative for HPV-testing in HPV-based screening programs in lower-resource contexts. | - |
dc.format.extent | 15 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0254946 | - |
dc.relation.ispartof | PLOS ONE, 2021, vol. 16, num. 7 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0254946 | - |
dc.rights | cc by (c) Hernández López, Rubí et al, 2021 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Papil·lomavirus | - |
dc.subject.classification | Anàlisi d'orina | - |
dc.subject.other | Papillomaviruses | - |
dc.subject.other | Urine--Analysis | - |
dc.title | Performance of an affordable urine self-sampling method for human papillomavirus detection in Mexican women | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2021-09-10T08:15:48Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 34288949 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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File | Description | Size | Format | |
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journal.pone.0254946.pdf | 1.02 MB | Adobe PDF | View/Open |
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