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Title: | Impact of the Epigenetically Regulated Hoxa-5 Gene in Neural Differentiation from Human Adipose-Derived Stem Cells |
Author: | Hernández Pérez, Rosa Jiménez Luna, Cristina Ortiz Quesada, Raúl Setién, Fernando López, Miguel Perazzoli, Gloria Esteller, Manel Berdasco, María Prados, Jose Melguizo, Consolación |
Keywords: | Teixit adipós Epigenètica Malalties del sistema nerviós Adipose tissues Nervous system Diseases Epigenetics |
Issue Date: | 19-Aug-2021 |
Publisher: | MDPI AG |
Abstract: | Human adipose-derived mesenchymal stem cells (hASCs) may be used in some nervous system pathologies, although obtaining an adequate degree of neuronal differentiation is an important barrier to their applicability. This requires a deep understanding of the expression and epigenetic changes of the most important genes involved in their differentiation. We used hASCs from human lipoaspirates to induce neuronal-like cells through three protocols (Neu1, 2, and 3), determined the degree of neuronal differentiation using specific biomarkers in culture cells and neurospheres, and analyzed epigenetic changes of genes involved in this differentiation. Furthermore, we selected the Hoxa-5 gene to determine its potential to improve neuronal differentiation. Our results showed that an excellent hASC neuronal differentiation process using Neu1 which efficiently modulated NES, CHAT, SNAP25, or SCN9A neuronal marker expression. In addition, epigenetic studies showed relevant changes in Hoxa-5, GRM4, FGFR1, RTEL1, METRN, and PAX9 genes. Functional studies of the Hoxa-5 gene using CRISPR/dCas9 and lentiviral systems showed that its overexpression induced hASCs neuronal differentiation that was accelerated with the exposure to Neu1. These results suggest that Hoxa-5 is an essential gene in hASCs neuronal differentiation and therefore, a potential candidate for the development of cell therapy strategies in neurological disorders. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/biology10080802 |
It is part of: | Biology, 2021, vol. 10, num. 8, p. 802 |
URI: | http://hdl.handle.net/2445/179993 |
Related resource: | https://doi.org/10.3390/biology10080802 |
ISSN: | 2079-7737 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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biology-10-00802-v2.pdf | 19.93 MB | Adobe PDF | View/Open |
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