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Title: Monitoring HOTTIP levels on extracellular vesicles for predicting recurrence in surgical non-small cell lung cancer patients.
Author: Han, Bing
Marrades Sicart, Ramon Ma.
Viñolas Segarra, Núria
He, Yangyi
Canals, Jordi
Díaz Sánchez, Tania
Molins López-Rodó, Laureano
Martinez, Daniel
Moisés, Jorge
Sánchez, David
Boada, Marc
Acosta Plasencia, Melissa
Cros Font, Coralí
Monzó Planella, Mariano
Navarro Ponz, Alfons
Keywords: Càncer de pulmó
Marcadors bioquímics
Lung cancer
Biochemical markers
Issue Date: 7-Jun-2021
Publisher: Elsevier
Abstract: In resected non-small cell lung cancer (NSCLC), postsurgical recurrence is the major factor affecting long-term survival. The identification of biomarkers in extracellular vesicles (EV) obtained from serial blood samples after surgery could enhance early detection of relapse and improve NSCLC outcome. Since EV cargo contains long non-coding RNAs (lncRNAs), we aimed to analyze whether the oncogenic lncRNA HOTTIP, which higher expression in tumor tissue was related to worse outcome in NSCLC, could be detected in EV from NSCLC patients and serve as recurrence biomarker. After purification of EVs by ultracentrifugation in 52 serial samples from 18 NSCLC patients, RNA was isolated and HOTTIP was quantified by Real time PCR. We observed that patients that relapsed after surgery displayed increased postsurgical EV HOTTIP levels in comparison with presurgical levels. In the relapsed patients with several samples available between surgery and relapse, we observed an increment in the EV HOTTIP levels when approaching to relapse, which indicated its potential utility for monitoring disease recurrence. When we focused in EV HOTTIP levels in the first post-surgical sample, we observed that the detection of an increment of the expression levels in comparison to presurgical sample, predicted recurrence with high sensitivity (85.7%) and specificity (90.9%) and that patients had shorter time to relapse and shorter overall survival. In conclusion, our pilot study showed that EV HOTTIP is a potential biomarker for monitoring disease recurrence after surgery in NSCLC.
Note: Reproducció del document publicat a:
It is part of: Translational Oncology, 2021, vol. 14, num. 8
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ISSN: 1944-7124
Appears in Collections:Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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