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http://hdl.handle.net/2445/181365
Title: | Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction |
Author: | Prat Vidal, Cristina Crisóstomo, Verónica Moscoso, Isabel Báez Díaz, Claudia Blanco Blázquez, Virginia Gómez Mauricio, Guadalupe Albericio, Guillermo Aguilar, Susana Fernández Santos, M. Eugenia Fernández Avilés, Francesc Sánchez Margallo, Francisco M. Bayés Genís, Antoni Bernad, Antonio |
Keywords: | Infart de miocardi Matriu extracel·lular Teràpia cel·lular Myocardial infarction Extracellular matrix Cellular therapy |
Issue Date: | 28-Sep-2021 |
Publisher: | MDPI AG |
Abstract: | Human cardiac progenitor cells (hCPC) are considered a good candidate in cell therapy for ischemic heart disease, demonstrating capacity to improve functional recovery after myocardial infarction (MI), both in small and large preclinical animal models. However, improvements are required in terms of cell engraftment and efficacy. Based on previously published reports, insulin-growth factor 1 (IGF-1) and hepatocyte growth factor (HGF) have demonstrated substantial cardioprotective, repair and regeneration activities, so they are good candidates to be evaluated in large animal model of MI. We have validated porcine cardiac progenitor cells (pCPC) and lentiviral vectors to overexpress IGF-1 (co-expressing eGFP) and HGF (co-expressing mCherry). pCPC were transduced and IGF1-eGFPpos and HGF-mCherrypos populations were purified by cell sorting and further expanded. Overexpression of IGF-1 has a limited impact on pCPC expression profile, whereas results indicated that pCPC-HGF-mCherry cultures could be counter selecting high expresser cells. In addition, pCPC-IGF1-eGFP showed a higher cardiogenic response, evaluated in co-cultures with decellularized extracellular matrix, compared with native pCPC or pCPC-HGF-mCherry. In vivo intracoronary co-administration of pCPC-IGF1-eGFP and pCPC-HFG-mCherry (1:1; 40 × 106/animal), one week after the induction of an MI model in swine, revealed no significant improvement in cardiac function. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/cells10102571 |
It is part of: | Cells, 2021, vol. 10, num. 10, p. 2571 |
URI: | http://hdl.handle.net/2445/181365 |
Related resource: | https://doi.org/10.3390/cells10102571 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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cells-10-02571.pdf | 4.76 MB | Adobe PDF | View/Open |
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