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http://hdl.handle.net/2445/181377
Title: | α-synuclein RT-QuIC in cerebrospinal fluid of LRRK2-linked Parkinson's disease |
Author: | Garrido, Alicia Fairfoul, Graham Tolosa, Eduardo Martí Domènech, Ma. Josep Green, Alison Ávila Rivera, Maria Asunción Barcelona LRRK2 Study Group |
Keywords: | Proteïnes quinases Malaltia de Parkinson Líquid cefalorraquidi Protein kinases Parkinson's disease Cerebrospinal fluid |
Issue Date: | 1-Jun-2019 |
Publisher: | American Neurological Association |
Abstract: | Background: leucine-rich kinase 2 (LRRK2)-linked Parkinson's disease (PD) is clinically indistinguishable from idiopathic PD (IPD). A pleiotropic neuropathology has been recognized but the majority of studies in LRRK2 p.G2019S patients reveal Lewy-type synucleinopathy as its principal histological substrate. To date no in vivo biomarkers of synucleinopathy have been found in LRRK2 mutation carriers. Objectives: we used real-time quaking-induced conversion (RT-QuIC) technique to assess the presence of alpha-synuclein (a-syn) aggregates in cerebrospinal fluid (CSF) of LRRK2 p.G2019S carriers. Methods: CSF samples of 51 subjects were analyzed: 15 LRRK2 p.G2019S PD, 10 IPD, 16 LRRK2 p.G2019S nonmanifesting carriers (NMC) and 10 healthy controls. The presence of parkinsonism and prodromal symptoms was assessed in all study subjects. Results: forty percent (n = 6) LRRK2-PD, and 18.8% (n = 3) LRRK2-NMC had a positive a-syn RT-QuIC response. RT-QuIC detected IPD with 90% sensitivity and 80% specificity. No clinical differences were detected between LRRK2-PD patients with positive and negative RT-QuIC. A positive RT-QuIC result in LRRK2-NMC occurred in a higher proportion of subjects meeting the Movement Disorder Society research criteria for prodromal PD. Interpretation: RT-QuIC detects a-syn aggregation in CSF in a significant number of patients with LRRK2-PD, but less frequently than in IPD. A small percentage of LRRK2-NMC tested also positive. If appropriately validated in long-term studies with large number of mutation carriers, and hopefully, postmortem or in vivo confirmation of histopathology, RT-QuIC could contribute to the selection of candidates to receive disease modifying drugs, in particular treatments targeting a-syn deposition. |
Note: | Reproducció del document publicat a: https://doi.org/10.1002/acn3.772 |
It is part of: | Annals of Clinical and Translational Neurology, 2019, vol. 6, num. 6, p. 1024-1032 |
URI: | http://hdl.handle.net/2445/181377 |
Related resource: | https://doi.org/10.1002/acn3.772 |
ISSN: | 2328-9503 |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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