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Title: Development of Lactoferrin-Loaded Liposomes for the Management of Dry Eye Disease and Ocular Inflammation
Author: López-Machado, Ana
Díaz Garrido, Natalia
Cano Fernández, Amanda
Espina García, Marta
Badía Palacín, Josefa
Baldomà Llavinés, Laura
Calpena Campmany, Ana Cristina
Souto, Eliana B.
García López, María Luisa
Sánchez-López, E. (Elena)
Keywords: Síndromes de l'ull sec
Sistemes d'alliberament de medicaments
Agents antiinflamatoris
Farmacologia ocular
Dry eye syndromes
Drug delivery systems
Antiinflammatory agents
Ocular pharmacology
Issue Date: 15-Oct-2021
Publisher: MDPI
Abstract: Abstract: Dry eye disease (DED) is a high prevalent multifactorial disease characterized by a lack of homeostasis of the tear film which causes ocular surface inflammation, soreness, and visual disturbance. Conventional ophthalmic treatments present limitations such as low bioavailability and side effects. Lactoferrin (LF) constitutes a promising therapeutic tool, but its poor aqueous stability and high nasolacrimal duct drainage hinder its potential efficacy. In this study, we incorporate lactoferrin into hyaluronic acid coated liposomes by the lipid film method, followed by high pressure homogenization. Pharmacokinetic and pharmacodynamic profiles were evaluated in vitro and ex vivo. Cytotoxicity and ocular tolerance were assayed both in vitro and in vivo using New Zealand rabbits, as well as dry eye and anti-inflammatory treatments. LF loaded liposomes showed an average size of 90 nm, monomodal population, positive surface charge and a high molecular weight protein encapsulation of 53%. Biopharmaceutical behaviour was enhanced by the nanocarrier, and any cytotoxic effect was studied in human corneal epithelial cells. Developed liposomes revealed the ability to reverse dry eye symptoms and possess anti-inflammatory efficacy, without inducing ocular irritation. Hence, lactoferrin loaded liposomes could offer an innovative nanotechnological tool as suitable approach in the treatment of DED.
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It is part of: Pharmaceutics, 2021, vol. 13, num. 10, p. 1698
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ISSN: 1999-4923
Appears in Collections:Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)
Articles publicats en revistes (Bioquímica i Fisiologia)

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