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http://hdl.handle.net/2445/182497
Title: | The hepatocyte nuclear factor 4 (HNF-4) represses the mitochondrial HMG-CoA synthase gene |
Author: | Rodríguez Rubio, Joan Carles Ortiz, José A. García Hegardt, Fausto Haro Bautista, Diego |
Keywords: | Àcids grassos Mitocondris Regulació del metabolisme Fatty acids Mitochondria Metabolic regulation |
Issue Date: | 1998 |
Publisher: | Elsevier B.V. |
Abstract: | We have recently shown that the gene for the mitochondrial HMG-CoA synthase is a target for PPAR and that this receptor mediates the induction of this gene by fatty acids. With the aim of gaining further insight into the function and regulation of this gene we examined the effect of other members of the nuclear hormone receptor superfamily on its expression. We previously identified a regulatory element in the mitochondrial HMG-CoA synthase gene promoter that confers transcriptional regulation by PPAR, RXR and the orphan nuclear receptor COUP-TF, In this study we demonstrate a trans-repressing regulatory function for HNF-4 at this same nuclear receptor response element (NRRE). HNF-4 binds to the mitochondrial HMG-CoA synthase NRRE, and, in cotransfection assays in HepG2 cells, it represses PPAR-dependent activation of a reporter gene linked to the mitochondrial HMG-CoA synthase gene promoter. These results suggest that the mitochondrial HMG-CoA synthase gene is subject to differential regulation by the interplay of multiple members of the nuclear hormone receptor superfamily. |
Note: | Versió postprint del document publicat a: https://www.sciencedirect.com/science/article/abs/pii/S0006291X97980323?via%3Dihub |
It is part of: | Biochemical and Biophysical Research Communications, 1998, vol. 242, num. 3, p. 692-696 |
URI: | http://hdl.handle.net/2445/182497 |
ISSN: | 0006-291X |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) |
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