Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/182931
Title: | MiR-125b downregulates macrophage scavenger receptor type B1 and reverse cholesterol transport |
Author: | Hueso, Miguel Griñán, Raquel Mallen, Adrián Navarro, Estanis Purqueras, Elvira Gomá, Montse Sbraga, Fabrizio Blasco Lucas, Arnau Revilla, Giovanna Santos, David Canyelles, Marina Julve, Josep Escolà Gil, Joan Carles Rotllan, Noemi |
Keywords: | Macròfags Colesterol Artèries coronàries Malalties coronàries Macrophages Cholesterol Coronary arteries Coronary diseases |
Issue Date: | 1-Feb-2022 |
Publisher: | Elsevier BV |
Abstract: | Objective: To determine whether miR-125b regulates cholesterol efflux in vivo and in vitro through the regulation of scavenger receptor type B1 (SR-B1). Approach and results: We demonstrated that miR-125b is up-regulated in the human aortas of patients with CAD and is located in macrophages and vascular smooth muscle cells (VSMCs). We identified SCARB1 as a direct target of miR-125b by repressing the activity of the SCARB1 3'-untranslated region reporter construct. Moreover, the overexpression of miR-125b in both human and mouse macrophages as well as VSMCs was found to downregulated the expression of the SCARB1 and the SR-B1 protein levels, thereby impairing alpha-HDL-mediated macrophage cholesterol efflux in vitro. The in vivo reverse cholesterol transport (RCT) rate from non-cholesterol-loaded macrophages transfected with miR-125b to feces was also found to be decreased when compared with that of control mimic-transfected macrophages. Conclusions: Together, these results provide evidence that miR-125b downregulates SCARB1 and SR-B1 in both human and mouse macrophages as well as VSMCs, thereby impairing macrophage cholesterol efflux in vitro and the whole macrophage-specific RCT pathway in vivo. |
Note: | Reproducció del document publicat a: https://doi.org/10.1016/j.biopha.2021.112596 |
It is part of: | Biomedicine & Pharmacotherapy, 2022, vol. 146, p. 112596 |
URI: | http://hdl.handle.net/2445/182931 |
Related resource: | https://doi.org/10.1016/j.biopha.2021.112596 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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