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http://hdl.handle.net/2445/183115
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DC Field | Value | Language |
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dc.contributor.author | Rodriguez Temporal, David | - |
dc.contributor.author | Alcaide Fernández de Vega, Fernando | - |
dc.contributor.author | Mareković, Ivana | - |
dc.contributor.author | O’Connor, James Anthony | - |
dc.contributor.author | Gorton, Rebecca | - |
dc.contributor.author | Ingen, Jakko van | - |
dc.contributor.author | Bossche, An van den | - |
dc.contributor.author | Héry-Arnaud, Genevieve | - |
dc.contributor.author | Beauruelle, Clémence | - |
dc.contributor.author | Orth-Höller, Dorothea | - |
dc.contributor.author | Palacios Gutiérrez, Juan José | - |
dc.contributor.author | Tudó i Vilanova, Griselda | - |
dc.contributor.author | Bou, Germán | - |
dc.contributor.author | Ceyssens, Pieter-Jan | - |
dc.contributor.author | Garrigó, Montserrat | - |
dc.contributor.author | González Martín, Julián | - |
dc.contributor.author | Greub, Gilbert | - |
dc.contributor.author | Hrabak, Jaroslav | - |
dc.contributor.author | Ingebretsen, André | - |
dc.contributor.author | Mediavilla Gradolph, Maria Concepción | - |
dc.contributor.author | Oviaño, Marina | - |
dc.contributor.author | Palop, Begoña | - |
dc.contributor.author | Pranada, Arthur B. | - |
dc.contributor.author | Quiroga, Lidia | - |
dc.contributor.author | Ruiz Serrano, Maria Jesús | - |
dc.contributor.author | Rodríguez Sánchez, Belén | - |
dc.date.accessioned | 2022-02-11T16:34:35Z | - |
dc.date.available | 2022-02-11T16:34:35Z | - |
dc.date.issued | 2022-01-24 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2445/183115 | - |
dc.description.abstract | The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Science and Business Media LLC | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/s41598-022-05315-7 | - |
dc.relation.ispartof | Scientific Reports, 2022, vol 12, num 1 | - |
dc.relation.uri | https://doi.org/10.1038/s41598-022-05315-7 | - |
dc.rights | cc by (c) Rodriguez Temporal, David et al, 2022 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Malalties del pulmó | - |
dc.subject.classification | Bacteris patògens | - |
dc.subject.other | Pulmonary diseases | - |
dc.subject.other | Pathogenic bacteria | - |
dc.title | Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2022-02-11T10:48:33Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 35075208 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (ISGlobal) Articles publicats en revistes (Fonaments Clínics) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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File | Description | Size | Format | |
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s41598-022-05315-7.pdf | 1.06 MB | Adobe PDF | View/Open |
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