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http://hdl.handle.net/2445/183268
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DC Field | Value | Language |
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dc.contributor.author | Tura-Ceide, Olga | - |
dc.contributor.author | Smolders, Valérie | - |
dc.contributor.author | Aventin, Núria | - |
dc.contributor.author | Morén Núñez, Constanza | - |
dc.contributor.author | Guitart Mampel, Mariona | - |
dc.contributor.author | Blanco Vich, Isabel | - |
dc.contributor.author | Piccari, Lucilla | - |
dc.contributor.author | Osorio, Jeisson | - |
dc.contributor.author | Rodríguez, Cristina | - |
dc.contributor.author | Rigol, Montserrat | - |
dc.contributor.author | Solanes, Núria | - |
dc.contributor.author | Malandrino, Andrea | - |
dc.contributor.author | Kurakula, Kondababu | - |
dc.contributor.author | Goumans, Marie José | - |
dc.contributor.author | Quax, Paul H. A. | - |
dc.contributor.author | Peinado Cabré, Víctor Ivo | - |
dc.contributor.author | Castellà, Manuel | - |
dc.contributor.author | Barberà i Mir, Joan Albert | - |
dc.date.accessioned | 2022-02-17T18:52:27Z | - |
dc.date.available | 2022-02-17T18:52:27Z | - |
dc.date.issued | 2021-09-22 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2445/183268 | - |
dc.description.abstract | Pulmonary endarterectomy (PEA) resected material offers a unique opportunity to develop an in vitro endothelial cell model of chronic thromboembolic pulmonary hypertension (CTEPH). We aimed to comprehensively analyze the endothelial function, molecular signature, and mitochondrial profile of CTEPH-derived endothelial cells to better understand the pathophysiological mechanisms of endothelial dysfunction behind CTEPH, and to identify potential novel targets for the prevention and treatment of the disease. Isolated cells from specimens obtained at PEA (CTEPH-EC), were characterized based on morphology, phenotype, and functional analyses (in vitro and in vivo tubule formation, proliferation, apoptosis, and migration). Mitochondrial content, morphology, and dynamics, as well as high-resolution respirometry and oxidative stress, were also studied. CTEPH-EC displayed a hyperproliferative phenotype with an increase expression of adhesion molecules and a decreased apoptosis, eNOS activity, migration capacity and reduced angiogenic capacity in vitro and in vivo compared to healthy endothelial cells. CTEPH-EC presented altered mitochondrial dynamics, increased mitochondrial respiration and an unbalanced production of reactive oxygen species and antioxidants. Our study is the foremost comprehensive investigation of CTEPH-EC. Modulation of redox, mitochondrial homeostasis and adhesion molecule overexpression arise as novel targets and biomarkers in CTEPH. | - |
dc.format.extent | 15 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/s41598-021-98320-1 | - |
dc.relation.ispartof | Scientific Reports, 2021, vol. 11, num. 1, p. 18797 | - |
dc.relation.uri | https://doi.org/10.1038/s41598-021-98320-1 | - |
dc.rights | cc-by (c) Tura-Ceide, Olga et al., 2021 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Articles publicats en revistes (Medicina) | - |
dc.subject.classification | Embòlia pulmonar | - |
dc.subject.classification | Endoteli | - |
dc.subject.classification | Hipertensió pulmonar | - |
dc.subject.other | Pulmonary embolism | - |
dc.subject.other | Endothelium | - |
dc.subject.other | Pulmonary hypertension | - |
dc.title | Derivation and characterisation of endothelial cells from patients with chronic thromboembolic pulmonary hypertension | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 719419 | - |
dc.date.updated | 2022-02-17T18:52:28Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/675527/EU//MOGLYNET | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Publicacions de projectes de recerca finançats per la UE Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Articles publicats en revistes (Medicina) |
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719419.pdf | 2.49 MB | Adobe PDF | View/Open |
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