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http://hdl.handle.net/2445/183314
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DC Field | Value | Language |
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dc.contributor.author | Zunino, Alessandro | - |
dc.contributor.author | Garzella, Francesco | - |
dc.contributor.author | Trianni, Alberta | - |
dc.contributor.author | Saggau, Peter | - |
dc.contributor.author | Bianchini, Paolo | - |
dc.contributor.author | Diaspro, Alberto | - |
dc.contributor.author | Duocastella, Martí | - |
dc.date.accessioned | 2022-02-18T09:24:14Z | - |
dc.date.available | 2022-02-18T09:24:14Z | - |
dc.date.issued | 2021-11-03 | - |
dc.identifier.issn | 2330-4022 | - |
dc.identifier.uri | http://hdl.handle.net/2445/183314 | - |
dc.description.abstract | Light-sheet microscopes have become the tool of choice for volumetric imaging of large samples. Based on a wide-field acquisition scheme, they are capable of optical sectioning at diffraction-limited resolution and minimal overall photodamage. Unfortunately, traditional architectures are limited in speed because 3D images are collected by either sample translation or synchronized movement of both light-sheet and detection objective lens. A promising solution avoiding slow mechanical movements is to extend the depth-of-field of the microscope and moving only the light-sheet. However, this normally comes at the cost of losing light and contrast, compromising the signal-to-noise ratio of the images. Here, we propose an innovative technique devoted to restoring the quality of the images, while preserving the speed of extended depth-of-field microscopes. It is based on generating a stack of parallel light-sheets using a pair of orthogonal acousto-optic deflectors, enabling the simultaneous illumination of different sample planes. Given the extended depth-of-field, all such planes appear in focus and can be acquired in a superimposed single frame. By applying a single-step inversion algorithm, we can decode a stack of frames into a volumetric image whose signal-to-noise ratio and contrast are greatly enhanced. We provide a detailed theoretical framework of the method and demonstrate its feasibility with volumetric images of kidney cell spheroids. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Chemical Society | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1021/acsphotonics.1c01401 | - |
dc.relation.ispartof | ACS Photonics, 2021 | - |
dc.relation.uri | https://doi.org/10.1021/acsphotonics.1c01401 | - |
dc.rights | (c) Zunino, Alessandro, et al., 2021 | - |
dc.source | Articles publicats en revistes (Física Aplicada) | - |
dc.subject.classification | Microscopis | - |
dc.subject.classification | Visualització tridimensional | - |
dc.subject.other | Microscopes | - |
dc.subject.other | Three-dimensional display systems | - |
dc.title | Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 716024 | - |
dc.date.updated | 2022-02-18T09:24:14Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Física Aplicada) |
Files in This Item:
File | Description | Size | Format | |
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716024.pdf | 4.02 MB | Adobe PDF | View/Open |
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