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Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/183522
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dc.contributor.authorCalon, Alexandre-
dc.contributor.authorEspinet, Elisa-
dc.contributor.authorPalomo Ponce, Sergio-
dc.contributor.authorTauriello, Daniele V. F.-
dc.contributor.authorIglesias, Mar-
dc.contributor.authorCéspedes, María-
dc.contributor.authorSevillano, Marta-
dc.contributor.authorNadal, Cristina-
dc.contributor.authorJung, Peter-
dc.contributor.authorZhang, Xiang-
dc.contributor.authorByrom, Daniel-
dc.contributor.authorRiera i Escalé, Antoni-
dc.contributor.authorRossell, David-
dc.contributor.authorMangues, Ramon-
dc.contributor.authorMassagué i Solé, Joan, 1953--
dc.contributor.authorSancho, Elena-
dc.contributor.authorBatlle, Eduard-
dc.date.accessioned2022-02-24T19:00:17Z-
dc.date.available2022-02-24T19:00:17Z-
dc.date.issued2014-05-05-
dc.identifier.issn2331-8325-
dc.identifier.urihttp://hdl.handle.net/2445/183522-
dc.description.abstractMetastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF- beta responses when injected into nude mice as shown by lack of p- SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we engineered CRC cell lines to secrete active TGF-beta. Subcutaneous tumors obtained from HT29-M6TGF-β, KM12L4aTGF-β cells and SW48TGF-β cells contained abundant p-SMAD2+ stromal cells.-
dc.format.extent4 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.21769/bioprotoc.1119-
dc.relation.ispartofBio-protocol, 2014, vol. 4, num. 9, p. e1119-
dc.relation.urihttps://doi.org/10.21769/bioprotoc.1119-
dc.rights(c) Bio-protocol LLC, 2014-
dc.sourceArticles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))-
dc.subject.classificationCàncer colorectal-
dc.subject.classificationMetàstasi-
dc.subject.classificationExpressió gènica-
dc.subject.otherColorectal cancer-
dc.subject.otherMetastasis-
dc.subject.otherGene expression-
dc.titleImmunostaining Protocol: P-Smad2 (Xenograft and Mice)-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.date.updated2022-02-24T12:05:08Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.idimarina6544613-
Appears in Collections:Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

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