1. Dipòsit Digital de la Universitat de Barcelona
  2. Recerca
  3. Institut de Recerca Biomèdica (IRB Barcelona)
  4. Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/183522
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCalon, Alexandre-
dc.contributor.authorEspinet, Elisa-
dc.contributor.authorPalomo Ponce, Sergio-
dc.contributor.authorTauriello, Daniele V. F.-
dc.contributor.authorIglesias, Mar-
dc.contributor.authorCéspedes, María-
dc.contributor.authorSevillano, Marta-
dc.contributor.authorNadal, Cristina-
dc.contributor.authorJung, Peter-
dc.contributor.authorZhang, Xiang-
dc.contributor.authorByrom, Daniel-
dc.contributor.authorRiera i Escalé, Antoni-
dc.contributor.authorRossell, David-
dc.contributor.authorMangues, Ramon-
dc.contributor.authorMassagué i Solé, Joan, 1953--
dc.contributor.authorSancho, Elena-
dc.contributor.authorBatlle, Eduard-
dc.date.accessioned2022-02-24T19:00:17Z-
dc.date.available2022-02-24T19:00:17Z-
dc.date.issued2014-05-05-
dc.identifier.issn2331-8325-
dc.identifier.urihttps://hdl.handle.net/2445/183522-
dc.description.abstractMetastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF- beta responses when injected into nude mice as shown by lack of p- SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we engineered CRC cell lines to secrete active TGF-beta. Subcutaneous tumors obtained from HT29-M6TGF-β, KM12L4aTGF-β cells and SW48TGF-β cells contained abundant p-SMAD2+ stromal cells.-
dc.format.extent4 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.21769/bioprotoc.1119-
dc.relation.ispartofBio-protocol, 2014, vol. 4, num. 9, p. e1119-
dc.relation.urihttps://doi.org/10.21769/bioprotoc.1119-
dc.rights(c) Bio-protocol LLC, 2014-
dc.sourceArticles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))-
dc.subject.classificationCàncer colorectal-
dc.subject.classificationMetàstasi-
dc.subject.classificationExpressió gènica-
dc.subject.otherColorectal cancer-
dc.subject.otherMetastasis-
dc.subject.otherGene expression-
dc.titleImmunostaining Protocol: P-Smad2 (Xenograft and Mice)-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.date.updated2022-02-24T12:05:08Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.idimarina6544613-
Appears in Collections:Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Files in This Item:
File Description SizeFormat 
Bio-protocol1119.pdf522.96 kBAdobe PDFView/Open
Show simple item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.