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http://hdl.handle.net/2445/184112
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DC Field | Value | Language |
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dc.contributor.author | Salaet, Ignasi | - |
dc.contributor.author | Marquès, Ramon | - |
dc.contributor.author | Yance Chávez, Tula del Carmen | - |
dc.contributor.author | Macías Vidal, Judit | - |
dc.contributor.author | Giménez Zaragoza, David | - |
dc.contributor.author | Aligué i Alemany, Rosa Maria | - |
dc.date.accessioned | 2022-03-14T17:53:17Z | - |
dc.date.available | 2022-03-14T17:53:17Z | - |
dc.date.issued | 2021-04-22 | - |
dc.identifier.issn | 0101-2061 | - |
dc.identifier.uri | http://hdl.handle.net/2445/184112 | - |
dc.description.abstract | The appA-So gene, encoding a phytase from Serratia odorifera, was cloned and heterologously expressed in Komagataella phaffii. The open reading frame of appA-So comprised 1281 bp that encoded a 426-amino acid protein, including a 27-amino acid signal peptide. The encoded phytase, AppA-So, showed 52% homology with other histidine acid phosphatases. The purified recombinant phytase showed optimal activity at 55 °C and pH 4.5, exhibiting enzymatic activity between pH 3.7 and 5.8, with a specific activity of 1123 U/mg at pH 4.5 and 37 °C. The AppA-So protein retained more than 85% of its initial activity after incubation in different pH conditions (pH 2.5-6.5) at 37 °C for 3 h. AppA-So activity was maintained over time and displayed a low Michaelis-Menten constant (Km) of 0.093 g/L. To the best of our knowledge, this is the first report of the cloning and characterization of the phytase from S. odorifera. Comparison of AppA-So with other well-known phytases suggests that the S. odorifera phytase has the lowest Km and highest stable activity over time, making it very suitable for use in the animal feed industry. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Sociedade Brasileira de Ciência e Tecnologia de Alimentos | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1021/acsfoodscitech.0c00074 | - |
dc.relation.ispartof | Food Science and Technology, 2021, vol. 1, p. 689-697 | - |
dc.rights | (c) Salaet, Ignasi et al., 2021 | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Clonatge | - |
dc.subject.classification | Biotecnologia alimentària | - |
dc.subject.other | Cloning | - |
dc.subject.other | Food biotechnology | - |
dc.title | Novel Long-Term Phytase from Serratia odorifera: Cloning, Expression, and Characterization. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 721487 | - |
dc.date.updated | 2022-03-14T17:53:18Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
Files in This Item:
File | Description | Size | Format | |
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721487.pdf | 3.64 MB | Adobe PDF | View/Open |
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