Please use this identifier to cite or link to this item:
Title: Electrochemical POC device for fast malaria quantitative diagnosis in whole blood by using magnetic beads, Poly-HRP and microfluidic paper electrodes
Author: Arias Alpízar, Kevin
Serna, Erica
Borgheti Cardoso, Livia Neves
Sulleiro, Elena
Fernandez Busquets, Xavier
Sánchez Montalvá, Adrián
Baldrich, Eva
Ruiz Vega, Gisela
Molina, Israel
Campo, Francisco Javier del
Keywords: Malària
Issue Date: 15-Feb-2020
Publisher: Elsevier
Citation: Arias-Alpízar K;de la Serna E;Borgheti-Cardoso L.N.;Sulleiro E;Fernandez-Busquets, X;Sánchez-Montalvá A;Baldrich E. Electrochemical POC device for fast malaria quantitative diagnosis in whole blood by using magnetic beads, Poly-HRP and microfluidic paper electrodes. Biosensors & Bioelectronics, 2020, 150, 111925
Abstract: © 2019 Elsevier B.V. Malaria, a parasitic infection caused by Plasmodium parasites and transmitted through the bite of infected female Anopheles mosquitos, is one of the main causes of mortality in many developing countries. Over 200 million new infections and nearly half a million deaths are reported each year, and more than three billion people are at risk of acquiring malaria worldwide. Nevertheless, most malaria cases could be cured if detected early. Malaria eradication is a top priority of the World Health Organisation. However, achieving this goal will require mass population screening and treatment, which will be hard to accomplish with current diagnostic tools. We report an electrochemical point-of-care device for the fast, simple and quantitative detection of Plasmodium falciparum lactate dehydrogenase (PfLDH) in whole blood samples. Sample analysis includes 5-min lysis to release intracellular parasites, and stirring for 5 more min with immuno-modified magnetic beads (MB) along with an immuno-modified signal amplifier. The rest of the magneto-immunoassay, including sample filtration, MB washing and electrochemical detection, is performed at a disposable paper electrode microfluidic device. The sensor provides PfLDH quantitation down to 2.47 ng mL−1 in spiked samples and for 0.006–1.5% parasitemias in Plasmodium-infected cultured red blood cells, and discrimination between healthy individuals and malaria patients presenting parasitemias >0.3%. Quantitative malaria diagnosis is attained with little user intervention, which is not achieved by other diagnostic methods.
Note: Reproducció del postprint publicat a:
It is part of: Biosensors & Bioelectronics, 2020, vol 150
Related resource:
ISSN: 0956-5663
Appears in Collections:Articles publicats en revistes (ISGlobal)
Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB))
Publicacions de projectes de recerca finançats per la UE
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

Files in This Item:
File Description SizeFormat 
2019_BiosensBioelect_ElectrochemicalPOC_RuizVega_Postprint.pdf2.07 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons