Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/184514
Title: | Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration? |
Author: | Bucchi, Cristina Ohlsson, Ella Anta i Vinyals, Josep Maria de Woelflick, Melanie Galler, Kerstin Manzanares Céspedes, María Cristina Widbiller, Matthias |
Keywords: | Cèl·lules mare Polpa dental Dentina Endodòncia Stem cells Dental pulp Dentin Endodontics |
Issue Date: | 2-Mar-2022 |
Publisher: | MDPI |
Abstract: | The aim of this study was to analyze the suitability of pluripotent stem cells derived from the amnion (hAECs) as a potential cell source for revitalization in vitro. hAECs were isolated from human placentas, and dental pulp stem cells (hDPSCs) and dentin matrix proteins (eDMPs) were obtained from human teeth. Both hAECs and hDPSCs were cultured with 10% FBS, eDMPs and an osteogenic differentiation medium (StemPro). Viability was assessed by MTT and cell adherence to dentin was evaluated by scanning electron microscopy. Furthermore, the expression of mineralization-, odontogenic differentiation- and epithelial-mesenchymal transition-associated genes was analyzed by quantitative real-time PCR, and mineralization was evaluated through Alizarin Red staining. The viability of hAECs was significantly lower compared with hDPSCs in all groups and at all time points. Both hAECs and hDPSCs adhered to dentin and were homogeneously distributed. The regulation of odontoblast differentiation- and mineralization-associated genes showed the lack of transition of hAECs into an odontoblastic phenotype; however, genes associated with epithelial-mesenchymal transition were significantly upregulated in hAECs. hAECs showed small amounts of calcium deposition after osteogenic differentiation with StemPro. Pluripotent hAECs adhere on dentin and possess the capacity to mineralize. However, they presented an unfavorable proliferation behavior and failed to undergo odontoblastic transition |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms23052830 |
It is part of: | International Journal of Molecular Sciences, 2022, vol. 23, num. 5, p. 2830 |
URI: | http://hdl.handle.net/2445/184514 |
Related resource: | https://doi.org/10.3390/ijms23052830 |
ISSN: | 1661-6596 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
722086.pdf | 3.81 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License