Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/184721
Title: Mycobacterial surface characters remodeled by growth conditions drive different tumor-infiltrating cells and systemic IFN-gamma/IL-17 release in bladder cancer treatment
Author: Guallar Garrido, Sandra
Campo Perez, Victor
Perez Trujillo, Miriam
Cabrera, Cecilia
Senserrich, Jordi
Sanchez Chardi, Alejandro
Rabanal Prados, Rosa Ma. (Rosa Maria)
Gomez Mora, Elisabet
Noguera Ortega, Estela
Luquin, Marina
Julian, Esther
Keywords: Càncer de bufeta
Immunoteràpia
Bladder cancer
Immunotheraphy
Issue Date: 23-Mar-2022
Abstract: The mechanism of action of intravesical Mycobacterium bovis BCG immunotherapy treatment for bladder cancer is not completely known, leading to misinterpretation of BCG-unresponsive patients, who have scarce further therapeutic options. BCG is grown under diverse culture conditions worldwide, which can impact the antitumor effect of BCG strains and could be a key parameter of treatment success. Here, BCG and the nonpathogenic Mycobacterium brumae were grown in four culture media currently used by research laboratories and BCG manufacturers: Sauton-A60, -G15 and -G60 and Middlebrook 7H10, and used as therapies in the orthotopic murine BC model. Our data reveal that each mycobacterium requires specific culture conditions to induce an effective antitumor response. since higher survival rates of tumor-bearing mice were achieved using M. brumae-A60 and BCG-G15 than the rest of the treatments. M. brumae-A60 was the most efficacious among all tested treatments in terms of mouse survival, cytotoxic activity of splenocytes against tumor cells, higher systemic production of IL-17 and IFN-gamma, and bladder infiltration of selected immune cells such as ILCs and CD4(TEM). BCG-G15 triggered an antitumor activity based on a massive infiltration of immune cells, mainly CD3(+) (CD4(+) and CD8(+)) T cells, together with high systemic IFN-gamma release. Finally, a reduced variety of lipids was strikingly observed in the outermost layer of M. brumae-A60 and BCG-G15 compared to the rest of the cultures, suggesting an influence on the antitumor immune response triggered. These findings contribute to understand how mycobacteria create an adequate niche to help the host subvert immunosuppressive tumor actions.
Note: Reproducció del document publicat a: https://doi.org/10.1080/2162402X.2022.2051845
It is part of: Oncoimmunology, 2022, vol 11, num 1
URI: http://hdl.handle.net/2445/184721
Related resource: https://doi.org/10.1080/2162402X.2022.2051845
ISSN: 2162-402X
6545032
Appears in Collections:Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

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