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http://hdl.handle.net/2445/184996
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DC Field | Value | Language |
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dc.contributor.author | Gay i Marín, Marina | - |
dc.contributor.author | Díaz Lobo, Mireia | - |
dc.contributor.author | Gusi Vives, Mar | - |
dc.contributor.author | Arauz-Garofalo, Gianluca | - |
dc.contributor.author | Vilanova, Mar | - |
dc.contributor.author | Giralt Lledó, Ernest | - |
dc.contributor.author | Vilaseca Casas, Marta | - |
dc.contributor.author | Guardiola Bagán, Salvador | - |
dc.date.accessioned | 2022-04-19T11:56:59Z | - |
dc.date.available | 2023-04-01T05:10:30Z | - |
dc.date.issued | 2022-04-01 | - |
dc.identifier.issn | 1439-7633 | - |
dc.identifier.uri | http://hdl.handle.net/2445/184996 | - |
dc.description.abstract | We report a quantitative proteomics data analysis pipeline which, coupled to protein-directed dynamic combinatorial chemistry (DDC) experiments, enables the rapid discovery and direct characterization of protein-protein interaction (PPI) modulators. A low-affinity PD-1 binder was incubated with a library of >100 D-peptides under thiol-exchange favoring conditions, in the presence of the target protein PD-1, and we determined the S-linked dimeric species that resulted amplified in the protein samples versus the controls. We chemically synthesized the target dimer candidates and validated them by thermophoresis binding and protein-protein interaction assays. The results provide a proof-of-concept for using this strategy in the high-throughput search of improved drug-like peptide binders that block therapeutically relevant protein-protein interactions. | ca |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | ca |
dc.publisher | Wiley-VCH | ca |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1002/cbic.202200152 | - |
dc.relation.ispartof | ChemBioChem, 2022 | - |
dc.relation.uri | https://doi.org/10.1002/cbic.202200152 | - |
dc.rights | (c) Wiley, 2022 | - |
dc.source | Articles publicats en revistes (Química Inorgànica i Orgànica) | - |
dc.subject.classification | Proteòmica | - |
dc.subject.classification | Termodinàmica | - |
dc.subject.other | Proteomics | - |
dc.subject.other | Thermodynamics | - |
dc.title | Proteomic tools for the quantitative analysis of artificial peptide libraries: detection and characterization of target-amplified PD-1 inhibitors | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.date.updated | 2022-04-19T11:26:32Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.idimarina | 6545111 | - |
dc.identifier.pmid | 35362647 | - |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
Files in This Item:
File | Description | Size | Format | |
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ChemBioChem_Guardiola_2022.pdf | 1.27 MB | Adobe PDF | View/Open |
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