Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/185442
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rodríguez Grande, Cristina | - |
dc.contributor.author | Hurtado, Juan Carlos | - |
dc.contributor.author | Rodríguez Maus, Sandra | - |
dc.contributor.author | Casas, Isaac | - |
dc.contributor.author | Castillo, Paola | - |
dc.contributor.author | Navarro, Mireia | - |
dc.contributor.author | Rakislova, Natalia | - |
dc.contributor.author | García-Basteiro, Alberto L. | - |
dc.contributor.author | Carrilho, Carla | - |
dc.contributor.author | Fernandes, Fabiola | - |
dc.contributor.author | Lovane, Lucilia | - |
dc.contributor.author | Jordao, Dercio | - |
dc.contributor.author | Ismail, Mamudo Rafik | - |
dc.contributor.author | Lorenzoni, Cesaltina | - |
dc.contributor.author | Cossa, Anelsio | - |
dc.contributor.author | Mandomando, Inácio | - |
dc.contributor.author | Bassat Orellana, Quique | - |
dc.contributor.author | Menéndez, Clara | - |
dc.contributor.author | Ordi i Majà, Jaume | - |
dc.contributor.author | Muñoz, Patricia | - |
dc.contributor.author | Pérez Lago, Laura | - |
dc.contributor.author | García de Viedma, Darío | - |
dc.contributor.author | Martínez Yoldi, Miguel Julián | - |
dc.date.accessioned | 2022-05-09T16:29:33Z | - |
dc.date.available | 2022-05-09T16:29:33Z | - |
dc.date.issued | 2021-06-11 | - |
dc.identifier.issn | 1198-743X | - |
dc.identifier.uri | http://hdl.handle.net/2445/185442 | - |
dc.description.abstract | Objectives: To characterize the clonal complexity in Mycobacterium tuberculosis (MTB) infections considering factors that help maximize the detection of coexisting strains/variants. Methods: Genotypic analysis by Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeats (MIRU-VNTR) was performed directly on 70 biopsy specimens from two or more different tissues involving 28 tuberculosis cases diagnosed post-mortem in Mozambique, a country with a high tuberculosis burden. Results: Genotypic data from isolates collected from two or more tissues were obtained for 23 of the 28 cases (82.1%), allowing the analysis of within-patient diversity. MIRU-VNTR analysis revealed clonal diversity in ten cases (35.7%). Five cases showed allelic differences in three or more loci, suggesting mixed infection with two different strains. In half of the cases showing within-host diversity, one of the specimens associated with clonal heterogeneity was brain tissue. Conclusions: Direct MTB genotyping from post-mortem tissue samples revealed a frequent within-host Mycobacterium tuberculosis diversity, including mixed and polyclonal infections. Most of this diversity would have been overlooked if only standard analysis of respiratory specimens had been performed. | - |
dc.format.extent | 5 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | European Society of Clinical Microbiology and Infectious Diseases | - |
dc.relation.isformatof | Reproduccío del document publicat a: https://doi.org/10.1016/j.cmi.2021.05.038 | - |
dc.relation.ispartof | Clinical Microbiology and Infection, 2021, vol. 27, num. 10, p. 1518 | - |
dc.relation.uri | https://doi.org/10.1016/j.cmi.2021.05.038 | - |
dc.rights | cc by (c) Rodríguez Grande, Cristina et al., 2021 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Mycobacterium tuberculosis | - |
dc.subject.classification | Àfrica oriental | - |
dc.subject.classification | Genètica bacteriana | - |
dc.subject.classification | Autòpsia | - |
dc.subject.classification | Anàlisi | - |
dc.subject.other | Mycobacterium tuberculosis | - |
dc.subject.other | East Africa | - |
dc.subject.other | Bacterial genetics | - |
dc.subject.other | Autopsy | - |
dc.subject.other | Assaying | - |
dc.title | High within-host diversity found from direct genotyping on post-mortem tuberculosis specimens in a high-burden setting | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 719525 | - |
dc.date.updated | 2022-05-09T16:29:33Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 34119641 | - |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
719525.pdf | 183.82 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License