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Title: Biofunctionalization with a TGF -1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in Beagle dogs
Author: Cirera, Andrea
Manzanares Céspedes, María Cristina
Sevilla, Pablo
Ortiz Hernández, Mónica
Galindo Moreno, Pablo
Gil, Javier
Keywords: Teixit ossi
Empelts ossis
Cirurgia dental
Experimentació animal
Bone grafting
Dental surgery
Animal experimentation
Issue Date: 27-Sep-2019
Publisher: MDPI
Abstract: Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial¿one untreated and another submitted to biofunctionalization with a TGF- 1 inhibitor peptide, P144, on dental alveolus. Materials and Methods: A synthetic bone graft was used, namely, (i) Maxresorb® (Botiss Klockner) (n = 12), and (ii) Maxresorb® (Botiss Klockner) biofunctionalized with P144 peptide (n = 12). Both bone grafts were implanted in the two hemimandibles of six beagle dogs in the same surgical time, immediately after tooth extraction. Two dogs were sacrificed 2, 4, and 8 weeks post implant insertion, respectively. The samples were submitted to histomorphometrical and histological analyses. For each sample, we quantified the new bone growth and the new bone formed around the biomaterial's granules. After optical microscopic histological evaluation, selected samples were studied using backscattered scanning electron microscopy (BS-SEM). Results: The biofunctionalization of the biomaterial's granules maintains a stable membranous bone formation throughout the experiment timeline, benefitting from the constant presence of vascular structures in the alveolar space, in a more active manner that in the control samples. Better results in the experimental groups were proven both by quantitative and qualitative analysis. Conclusions: Synthetic bone graft biofunctionalization results in slightly better quantitative parameters of the implant's osseointegration. The qualitative histological and ultramicroscopic analysis shows that biofunctionalization may shorten the healing period of dental biomaterials.
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It is part of: Materials, 2019, vol. 12, num. 19, p. 3168
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ISSN: 1996-1944
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

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