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http://hdl.handle.net/2445/186050
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DC Field | Value | Language |
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dc.contributor.author | Fernández-Huerta, Miguel | - |
dc.contributor.author | Moreto, Clara | - |
dc.contributor.author | Vila-Olmo, Neus | - |
dc.contributor.author | García de Cara, Erika Inés | - |
dc.contributor.author | Basaez, Celeste | - |
dc.contributor.author | Santín Cerezales, Miguel | - |
dc.contributor.author | Alcaide Fernández de Vega, Fernando | - |
dc.date.accessioned | 2022-05-26T15:28:26Z | - |
dc.date.available | 2022-05-26T15:28:26Z | - |
dc.date.issued | 2021-07-19 | - |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.uri | http://hdl.handle.net/2445/186050 | - |
dc.description.abstract | Diagnosis of latent tuberculosis infection (LTBI) is considered key in the control of tuberculosis. Interferon gamma (IFN-g) release assays, such as the QuantiFERON-TB Gold Plus test (QFT-Plus), are now widely implemented for the in vitro diagnosis of LTBI. To date, the detection and quantification of IFN-g has been mostly performed with semiautomated enzyme-linked immunosorbent assays (ELISAs), but several limitations currently exist. The study aims to evaluate the chemiluminescence immunoassay (CLIA) analyzer Liaison XL compared to ELISA for the performance of the QFT-Plus test. Between February and April 2020, 333 heparin blood samples from 323 adult patients were collected at a tertiary teaching hospital in Barcelona, Spain. Overall, the CLIA analyzer Liaison XL performed well for the detection of IFN-g compared to the ELISA method, demonstrating substantial agreement (κ, 0.872) and great correlation between assays (r, .0.950). CLIA produced significantly higher values of IFN-g IU per milliliter than the ELISA (P = 0.004 for the TB1 tube and P = 0.010 for the TB2 tube). Many discrepant cases (8/15, 53.3%) corresponded to indeterminate results with ELISA (NIL-corrected mitogen value of ,0.5 IU/ml), which, when analyzed with the CLIA analyzer Liaison XL, reverted to interpretable results. In conclusion, this analysis suggests that CLIA presents a greater sensitivity for the identification of LTBI, especially among immunocompromised patients. Furthermore, the analytical variability reported between both ELISA and CLIA methods, especially around the standardized 0.35-IU/ml positivity threshold, suggests the need to refine the interpretative algorithm. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1128/JCM.00603-21 | - |
dc.relation.ispartof | Journal of Clinical Microbiology, 2021, vol. 59, num. 8, p. e0060321 | - |
dc.relation.uri | https://doi.org/10.1128/JCM.00603-21 | - |
dc.rights | (c) American Society for Microbiology, 2021 | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Tuberculosi | - |
dc.subject.classification | Anàlisi de sang | - |
dc.subject.classification | Luminescència | - |
dc.subject.classification | Interferó | - |
dc.subject.other | Tuberculosis | - |
dc.subject.other | Analysis of blood | - |
dc.subject.other | Luminescence | - |
dc.subject.other | Interferon | - |
dc.title | Evaluation of the fully automated chemiluminescence analyzer Liaison XL for the performance of the QuantiFERON-TB Gold Plus assay in an area with a low incidence of tuberculosis | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 716159 | - |
dc.date.updated | 2022-05-26T15:28:27Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 34076474 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
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716159.pdf | 387.54 kB | Adobe PDF | View/Open |
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