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http://hdl.handle.net/2445/186239
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DC Field | Value | Language |
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dc.contributor.author | Vallejo-Gracia, Albert | - |
dc.contributor.author | Sastre, Daniel | - |
dc.contributor.author | Colomer-Molera, Magalí | - |
dc.contributor.author | Solé, Laura | - |
dc.contributor.author | Navarro-Pérez, María | - |
dc.contributor.author | Capera Aragonés, Jesusa | - |
dc.contributor.author | Roig, Sara R. | - |
dc.contributor.author | Pedrós-Gámez, Oriol | - |
dc.contributor.author | Estadella, Irene | - |
dc.contributor.author | Szilágyi, Orsolya | - |
dc.contributor.author | Panyi, Gyorgy | - |
dc.contributor.author | Hajdú, Péter | - |
dc.contributor.author | Felipe Campo, Antonio | - |
dc.date.accessioned | 2022-06-01T16:43:21Z | - |
dc.date.available | 2022-06-01T16:43:21Z | - |
dc.date.issued | 2021-07-16 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2445/186239 | - |
dc.description.abstract | The voltage-dependent potassium channel Kv1.3 plays essential roles in the immune system, participating in leukocyte activation, proliferation and apoptosis. The regulatory subunit KCNE4 acts as an ancillary peptide of Kv1.3, modulates K+ currents and controls channel abundance at the cell surface. KCNE4-dependent regulation of the oligomeric complex fne-tunes the physiological role of Kv1.3. Thus, KCNE4 is crucial for Ca2+-dependent Kv1.3-related leukocyte functions. To better understand the role of KCNE4 in the regulation of the immune system, we manipulated its expression in various leukocyte cell lines. Jurkat T lymphocytes exhibit low KCNE4 levels, whereas CY15 dendritic cells, a model of professional antigen-presenting cells, robustly express KCNE4. When the cellular KCNE4 abundance was increased in T cells, the interaction between KCNE4 and Kv1.3 afected important T cell physiological features, such as channel rearrangement in the immunological synapse, cell growth, apoptosis and activation, as indicated by decreased IL-2 production. Conversely, ablation of KCNE4 in dendritic cells augmented proliferation. Furthermore, the LPS-dependent activation of CY15 cells, which induced Kv1.3 but not KCNE4, increased the Kv1.3-KCNE4 ratio and increased the expression of free Kv1.3 without KCNE4 interaction. Our results demonstrate that KCNE4 is a pivotal regulator of the Kv1.3 channelosome, which fne-tunes immune system physiology by modulating Kv1.3-associated leukocyte functions. | - |
dc.format.extent | 14 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/s41598-021-94015-9 | - |
dc.relation.ispartof | Scientific Reports, 2021, vol. 11, p. 14632 | - |
dc.relation.uri | https://doi.org/10.1038/s41598-021-94015-9 | - |
dc.rights | cc-by (c) Vallejo-Gracia, Albert et al., 2021 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) | - |
dc.subject.classification | Canals de potassi | - |
dc.subject.classification | Sistema immunitari | - |
dc.subject.classification | Leucòcits | - |
dc.subject.classification | Cèl·lules T | - |
dc.subject.other | Potassium channels | - |
dc.subject.other | Immune system | - |
dc.subject.other | Leucocytes | - |
dc.subject.other | T cells | - |
dc.title | KCNE4-dependent functional consequences of Kv1.3-related leukocyte physiology. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 715777 | - |
dc.date.updated | 2022-06-01T16:43:22Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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715777.pdf | 2.4 MB | Adobe PDF | View/Open |
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