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http://hdl.handle.net/2445/186609
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DC Field | Value | Language |
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dc.contributor.author | Hoijman Kleinman, Esteban | - |
dc.contributor.author | Rocha Viegas, Luciana | - |
dc.contributor.author | Keller Sarmiento, María Inés | - |
dc.contributor.author | Rosenstein, Ruth E. | - |
dc.contributor.author | Pecci, Adali | - |
dc.date.accessioned | 2022-06-14T15:50:50Z | - |
dc.date.available | 2022-06-14T15:50:50Z | - |
dc.date.issued | 2004-01 | - |
dc.identifier.issn | 0013-7227 | - |
dc.identifier.uri | http://hdl.handle.net/2445/186609 | - |
dc.description.abstract | The antiapoptotic effect of melatonin has been described in several systems. In this study, the antagonistic effect of the methoxyindole on dexamethasone-induced apoptosis in mouse thymocytes was examined. Melatonin decreased both DNA fragmentation, and the number of annexin V-positive cells incubated in the presence of dexamethasone. Analysis of the expression of the members of the Bcl-2 family indicated that the synthetic glucocorticoid increased Bax protein levels without affecting the levels of Bcl-2, Bcl-XL, Bcl-XS, or Bak. This effect correlated with an increase in thymocytes bax mRNA levels. Dexamethasone also increased the release of cytochrome C from mitochondria. All of these effects were reduced in the presence of melatonin, which was ineffective per se on these parameters. In addition, the involvement of cAMP on glucocorticoid/melatonin antagonism was examined. Both melatonin and dexamethasone decreased the levels of this nucleotide in mouse thymocytes, indicating that the antagonistic action between both hormones involves a cAMP-independent pathway. In summary, the present results suggest that the antiapoptotic effect of melatonin on glucocorticoid-treated thymocytes would be a consequence of an inhibition of the mitochondrial pathway, presumably through the regulation of Bax protein levels. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Association for the Study of Internal Secretions | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1210/en.2003-0764 | - |
dc.relation.ispartof | Endocrinology, 2004, vol. 145, num. 1, p. 418-425 | - |
dc.relation.uri | https://doi.org/10.1210/en.2003-0764 | - |
dc.rights | (c) Association for the Study of Internal Secretions, 2004 | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Apoptosi | - |
dc.subject.classification | Melatonina | - |
dc.subject.classification | Glucocorticoides | - |
dc.subject.classification | Proteïnes | - |
dc.subject.other | Apoptosis | - |
dc.subject.other | Melatonin | - |
dc.subject.other | Glucocorticoids | - |
dc.subject.other | Proteins | - |
dc.title | Involvement of Bax protein in the prevention of glucocorticoid-induced thymocytes apoptosis by melatonin | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 715246 | - |
dc.date.updated | 2022-06-14T15:50:51Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 14500572 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) |
Files in This Item:
File | Description | Size | Format | |
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715246.pdf | 1 MB | Adobe PDF | View/Open |
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