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http://hdl.handle.net/2445/18663
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DC Field | Value | Language |
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dc.contributor.author | Tailor, A. | cat |
dc.contributor.author | Tomlinson, Annette, 1942- | cat |
dc.contributor.author | Salas Martínez, Azucena | cat |
dc.contributor.author | Panés Díaz, Julià | cat |
dc.contributor.author | Granger, D. Neil | cat |
dc.contributor.author | Flower, Roderick J. | cat |
dc.contributor.author | Perretti, M. | cat |
dc.date.accessioned | 2011-07-07T12:30:40Z | - |
dc.date.available | 2011-07-07T12:30:40Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0017-5749 | - |
dc.identifier.uri | http://hdl.handle.net/2445/18663 | - |
dc.description.abstract | BACKGROUND A previous study showed that the glucocorticoid dexamethasone, at doses of 100 ¿g/kg and above, inhibited leucocyte adhesion to rat mesenteric postcapillary venules activated with interleukin 1ß (IL-1ß), as assessed by videomicroscopy. AIMS To identify whether the adhesion molecule, intercellular adhesion molecule 1 (ICAM-1), or the chemokine KC could be targeted by the steroid to mediate its antiadhesive effect. METHODS Rat mesenteries were treated with IL-1ß (20 ng intraperitoneally) and the extent of leucocyte adhesion measured at two and four hours using intravital microscopy. Rats were treated with dexamethasone, and passively immunised against ICAM-1 or KC. Endogenous expression of these two mediators was validated by immunohistochemistry, ELISA, and the injection of specific radiolabelled antibodies. RESULTS Dexamethasone greatly reduced IL-1ß induced leucocyte adhesion, endothelial expression of ICAM-1 in the postcapillary venule, and release of the mast cell derived chemokine KC. Injection of specific antibodies to the latter mediators was also extremely effective in downregulating (>80%) IL-1ß induced leucocyte adhesion. CONCLUSIONS Induction by IL-1ß of endogenous ICAM-1 and KC contributes to leucocyte adhesion to inflamed mesenteric vessels. Without excluding other possible mediators, these data clearly show that dexamethasone interferes with ICAM-1 expression and KC release from mast cells, resulting in suppression of leucocyte accumulation in the bowel wall, which is a prominent feature of several gastrointestinal pathologies. | eng |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | eng |
dc.publisher | BMJ Group | eng |
dc.relation.isformatof | Reproducció digital del document publicat a: http://dx.doi.org/10.1136/gut.45.5.705 | cat |
dc.relation.ispartof | Gut, 1999, vol. 45, núm. 5, p. 705-712 | - |
dc.relation.uri | http://dx.doi.org/10.1136/gut.45.5.705 | - |
dc.rights | (c) BMJ Publishing Group Ltd and British Society of Gastroenterology, 1999 | - |
dc.source | Articles publicats en revistes (Medicina) | - |
dc.subject.classification | Biologia molecular | cat |
dc.subject.classification | Leucòcits | cat |
dc.subject.other | Molecular biology | eng |
dc.subject.other | Leukocytes | eng |
dc.title | Dexamethasone inhibition of leucocyte adhesion to rat mesenteric postcapillary venules: role of intercellular adhesion molecule 1 and KC | eng |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 551417 | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 10517906 | - |
Appears in Collections: | Articles publicats en revistes (Medicina) |
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File | Description | Size | Format | |
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551417.pdf | 167.59 kB | Adobe PDF | View/Open |
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