Please use this identifier to cite or link to this item:
https://hdl.handle.net/2445/186734
Title: | Müller glia fused with adult stem cells undergo neural differentiation in human retinal models |
Author: | Bonilla Pons, Sergi Àngel Nakagawa, Shoma Garreta, Elena Fernández Blanco, Álvaro Pesaresi, Martina Antin, Justin Christopher d' Sebastian Perez, Ruben Greco, Daniela Domínguez Sala, Eduardo Gómez Riera, Raúl Barraquer Compte, Rafael Igancio Dierssen, Mara Montserrat Pulido, Núria Cosma, Maria Pia |
Keywords: | Trastorns de la visió Medicina regenerativa Vision disorders Regenerative medicine |
Issue Date: | 9-Mar-2022 |
Publisher: | Elsevier |
Abstract: | Visual impairments are a critical medical hurdle to be addressed in modern society. Müller glia (MG) have regenerative potential in the retina in lower vertebrates, but not in mammals. However, in mice, in vivo cell fusion between MG and adult stem cells forms hybrids that can partially regenerate ablated neurons.We used organotypic cultures of human retina and preparations of dissociated cells to test the hypothesis that cell fusion between human MG and adult stem cells can induce neuronal regeneration in human systems. Moreover, we established a microinjection system for transplanting human retinal organoids to demonstrate hybrid differentiation.We first found that cell fusion occurs between MG and adult stem cells, in organotypic cultures of human retina as well as in cell cultures. Next, we showed that the resulting hybrids can differentiate and acquire a proto-neural electrophysiology profile when the Wnt/beta-catenin pathway is activated in the adult stem cells prior fusion. Finally, we demonstrated the engraftment and differentiation of these hybrids into human retinal organoids.We show fusion between human MG and adult stem cells, and demonstrate that the resulting hybrid cells can differentiate towards neural fate in human model systems. Our results suggest that cell fusion-mediated therapy is a potential regenerative approach for treating human retinal dystrophies.This work was supported by La Caixa Health (HR17-00231), Velux Stiftung (976a) and the Ministerio de Ciencia e Innovación, (BFU2017-86760-P) (AEI/FEDER, UE), AGAUR (2017 SGR 689, 2017 SGR 926).Published by Elsevier B.V. |
Note: | Reproducció del document publicat a: https://doi.org/10.1016/j.ebiom.2022.103914 |
It is part of: | Ebiomedicine, 2022, vol. 77 |
URI: | https://hdl.handle.net/2445/186734 |
Related resource: | https://doi.org/10.1016/j.ebiom.2022.103914 |
ISSN: | 2352-3964 |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
2022_eBioMed_Muller_MontserratN.pdf | 4.24 MB | Adobe PDF | View/Open |
This item is licensed under a
Creative Commons License