Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/187393
Title: Molecular basis or arginine and lysine DNA sequence-dependent thermo-stability modulation
Author: Martín Martínez, Benjamín
Dans, Pablo D.
Wieczór, Miłosz
Villegas, Núria
Brun-Heath, Isabelle
Battistini, Federica
Terrazas Martínez, Montserrat
Orozco López, Modesto
Keywords: ADN
Biologia molecular
Termodinàmica
Aminoàcids
DNA
Molecular biology
Thermodynamics
Amino acids
Issue Date: 10-Jan-2022
Publisher: Public Library of Science (PLoS)
Abstract: We have used a variety of theoretical and experimental techniques to study the role of four basic amino acids-Arginine, Lysine, Ornithine and L-2,4-Diaminobutyric acid-on the structure, flexibility and sequence-dependent stability of DNA. We found that the presence of organic ions stabilizes the duplexes and significantly reduces the difference in stability between AT- and GC-rich duplexes with respect to the control conditions. This suggests that these amino acids, ingredients of the primordial soup during abiogenesis, could have helped to equalize the stability of AT- and GC-rich DNA oligomers, facilitating a general noncatalysed self-replication of DNA. Experiments and simulations demonstrate that organic ions have an effect that goes beyond the general electrostatic screening, involving specific interactions along the grooves of the double helix. We conclude that organic ions, largely ignored in the DNA world, should be reconsidered as crucial structural elements far from mimics of small inorganic cations.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pcbi.1009749
It is part of: PLoS Computational Biology, 2022, vol. 18, num. 1, p. e1009749
URI: http://hdl.handle.net/2445/187393
Related resource: https://doi.org/10.1371/journal.pcbi.1009749
ISSN: 1553-734X
Appears in Collections:Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
Articles publicats en revistes (Química Inorgànica i Orgànica)

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