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|Preliminary study for simultaneous detection and quantification of androgenic anabolic steroids using ELISA and pattern recognition techniques
Salvador, J. Pablo
Marco, M. Pilar
Marco Colás, Santiago
|Enzimoimmunoassaig sobre fase sòlida
Enzyme-linked immunosorbent assay
|Royal Society of Chemistry
|A first step towards the multidetection, identification and quantification of anabolic androgenic steroids by enzyme-linked immunosorbent assay (ELISA) has been performed in this study. This proposal combines multiple competitive ELISA assays with different cross-reactivity profiles and multivariate data analysis techniques. Data have been analyzed by principal component analysis in conjunction with a novel K-nearest line classifier. This proposal allows simultaneous detection of up to four different steroids in the range of concentration from 0.1 to 316.2 nM with a total rate of 90.6% of correct detection, even in the presence of cross-reactivities. A methodology for concentration prediction is also presented with satisfactory results.
|Versió postprint del document publicat a: https://doi.org/10.1039/C1AN15114B
|It is part of:
|Analyst, 2011, vol. 136, num. 19, p. 4045-4052
|Appears in Collections:
|Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
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