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Title: Preliminary study for simultaneous detection and quantification of androgenic anabolic steroids using ELISA and pattern recognition techniques
Author: Calvo, Daniel
Tort, Núria
Salvador, J. Pablo
Marco, M. Pilar
Centi, Fabiana
Marco Colás, Santiago
Keywords: Enzimoimmunoassaig sobre fase sòlida
Enzyme-linked immunosorbent assay
Issue Date: 8-Aug-2011
Publisher: Royal Society of Chemistry
Abstract: A first step towards the multidetection, identification and quantification of anabolic androgenic steroids by enzyme-linked immunosorbent assay (ELISA) has been performed in this study. This proposal combines multiple competitive ELISA assays with different cross-reactivity profiles and multivariate data analysis techniques. Data have been analyzed by principal component analysis in conjunction with a novel K-nearest line classifier. This proposal allows simultaneous detection of up to four different steroids in the range of concentration from 0.1 to 316.2 nM with a total rate of 90.6% of correct detection, even in the presence of cross-reactivities. A methodology for concentration prediction is also presented with satisfactory results.
Note: Versió postprint del document publicat a:
It is part of: Analyst, 2011, vol. 136, num. 19, p. 4045-4052
Related resource:
ISSN: 0003-2654
Appears in Collections:Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

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