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http://hdl.handle.net/2445/188943
Title: | Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variables |
Author: | Plata Peña, Laura López Rodrigo, Olga Bassas, Lluís Larriba, Sara |
Keywords: | Marcadors bioquímics Andrologia Vesícules seminals Biochemical markers Andrology Seminal vesicles |
Issue Date: | 3-Sep-2022 |
Publisher: | Wiley |
Abstract: | Background and objectives: Predicting the origin of azoospermia with non-invasive biomarkers is clinically relevant for determining the chance of successful sperm retrieval from the testes before attempting ART. Here, the semen small extracellular vesicle (sEV) microRNA (miRNA) miR-31-5p-based biomarker test to distinguish obstructive (OA) from secretory azoospermia (SA) (previously described by our group) is validated for clinical use and additionally sample source [seminal sEVs vs total seminal plasma (SP)] as a preanalytical variable is considered to optimize the procedure. Results and discussion: Our results provide evidence that altered miR-31-5p expression can be determined both from EVs and from the whole SP to discriminate OA from SA azoospermic samples. Not only have we validated this miRNA-based molecular model as a clinically useful test for predicting the origin of azoospermia in a sample from azoospermic individuals, but additionally, and more interestingly for the clinicians, we have evidenced its usefulness for predicting the presence of spermatogenic failure in azoospermic patients with FSH values <10 IU/L as a sensitive and specific biomarker (AUC>0.88; p-value<0.006). This article is protected by copyright. All rights reserved. Keywords: Seminal plasma; azoospermia; extracellular vesicles; miRNA; non-invasive biomarker. |
Note: | versió postprint del document publicat a: https://doi.org/10.1111/andr.13286 |
It is part of: | Andrology, 2022 |
URI: | http://hdl.handle.net/2445/188943 |
Related resource: | https://doi.org/10.1111/andr.13286 |
ISSN: | 2047-2927 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
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R2_Texto_Optimización test_paper.pdf | 683.84 kB | Adobe PDF | View/Open |
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