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Title: Nanoscale alterations in GABAB receptors and GIRK channel organization on the hippocampus of APP/PS1 mice
Author: Martín Belmonte, Alejandro
Aguado, Carolina
Alfaro Ruiz, Rocío
Moreno Martínez, Ana Esther
De La Ossa, Luis
Aso Pérez, Ester
Gómez Acero, Laura
Shigemoto, Ryuichi
Fukazawa, Yugo
Ciruela Alférez, Francisco
Luján, Rafael
Keywords: Hipocamp (Cervell)
Hippocampus (Brain)
Issue Date: 21-Sep-2022
Publisher: Springer Science and Business Media LLC
Abstract: Alzheimer's disease (AD) is characterized by a reorganization of brain activity determining network hyperexcitability and loss of synaptic plasticity. Precisely, a dysfunction in metabotropic GABA(B) receptor signalling through G protein-gated inwardly rectifying K+ (GIRK or Kir3) channels on the hippocampus has been postulated. Thus, we determined the impact of amyloid-beta (A beta) pathology in GIRK channel density, subcellular distribution, and its association with GABA(B) receptors in hippocampal CA1 pyramidal neurons from the APP/PS1 mouse model using quantitative SDS-digested freeze-fracture replica labelling (SDS-FRL) and proximity ligation in situ assay (P-LISA). In wild type mice, single SDS-FRL detection revealed a similar dendritic gradient for GIRK1 and GIRK2 in CA1 pyramidal cells, with higher densities in spines, and GIRK3 showed a lower and uniform distribution. Double SDS-FRL showed a co-clustering of GIRK2 and GIRK1 in post- and presynaptic compartments, but not for GIRK2 and GIRK3. Likewise, double GABA(B1) and GIRK2 SDS-FRL detection displayed a high degree of co-clustering in nanodomains (40-50 nm) mostly in spines and axon terminals. In APP/PS1 mice, the density of GIRK2 and GIRK1, but not for GIRK3, was significantly reduced along the neuronal surface of CA1 pyramidal cells and in axon terminals contacting them. Importantly, GABA(B1) and GIRK2 co-clustering was not present in APP/PS1 mice. Similarly, P-LISA experiments revealed a significant reduction in GABA(B1) and GIRK2 interaction on the hippocampus of this animal model. Overall, our results provide compelling evidence showing a significant reduction on the cell surface density of pre- and postsynaptic GIRK1 and GIRK2, but not GIRK3, and a decline in GABA(B) receptors and GIRK2 channels co-clustering in hippocampal pyramidal neurons from APP/PS1 mice, thus suggesting that a disruption in the GABA(B) receptor-GIRK channel membrane assembly causes dysregulation in the GABA(B) signalling via GIRK channels in this AD animal model.
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It is part of: Alzheimer's Research & Therapy, 2022, vol. 14, núm. 1
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ISSN: 1758-9193
Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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