Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/191121
Title: The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency
Author: Grande, Giuseppe
Barrachina, Ferran
Soler Ventura, Ada
Jodar Bifet, Meritxell
Mancini, Francesca Romana
Marana, Riccardo
Chiloiro, Sabrina
Pontecorvi, Alfredo
Oliva Virgili, Rafael
Milardi, Domenico
Keywords: Espermatozoides
Testosterona
Proteòmica
Hipogonadisme
Hormona luteostimulant
Spermatozoa
Testosterone
Proteomics
Hypogonadism
Luteinizing hormone
Issue Date: 19-May-2022
Publisher: Frontiers Media
Abstract: Testosterone is essential to maintain qualitative spermatogenesis. Nonetheless, no studies have been yet performed in humans to analyze the testosterone-mediated expression of sperm proteins and their importance in reproduction. Thus, this study aimed to identify sperm protein alterations in male hypogonadism using proteomic profiling. We have performed a comparative proteomic analysis comparing sperm from fertile controls (a pool of 5 normogonadic normozoospermic fertile men) versus sperm from patients with secondary hypogonadism (a pool of 5 oligozoospermic hypogonadic patients due to isolated LH deficiency). Sperm protein composition was analyzed, after peptide labelling with Isobaric Tags, via liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) on an LTQ Velos-Orbitrap mass spectrometer. LC-MS/MS data were analyzed using Proteome Discoverer. Criteria used to accept protein identification included a false discovery rate (FDR) of 1% and at least 1 peptide match per protein. Up to 986 proteins were identified and, of those, 43 proteins were differentially expressed: 32 proteins were under-expressed and 11 were over-expressed in the pool of hypogonadic patients compared to the controls. Bioinformatic analyses were performed using UniProt Knowledgebase, and the Gene Ontology Consortium database based on PANTHER. Notably, 13 of these 43 differentially expressed proteins have been previously reported to be related to sperm function and spermatogenesis. Western blot analyses for A-Kinase Anchoring Protein 3 (AKAP3) and the Prolactin Inducible Protein (PIP) were used to confirm the proteomics data. In summary, a high-resolution mass spectrometry-based proteomic approach was used for the first time to describe alterations of the sperm proteome in secondary male hypogonadism. Some of the differential sperm proteins described in this study, which include Prosaposin, SMOC-1, SERPINA5, SPANXB1, GSG1, ELSPBP1, fibronectin, 5-oxoprolinase, AKAP3, AKAP4, HYDIN, ROPN1B, ß-Microseminoprotein and Protein S100-A8, could represent new targets for the design of infertility treatments due to androgen deficiency.
Note: Reproducció del document publicat a: https://doi.org/10.3389/fendo.2022.852661
It is part of: Frontiers In Endocrinology, 2022, vol. 13, p. 852661
URI: http://hdl.handle.net/2445/191121
Related resource: https://doi.org/10.3389/fendo.2022.852661
ISSN: 1664-2392
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Biomedicina)

Files in This Item:
File Description SizeFormat 
725933.pdf892.01 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons