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http://hdl.handle.net/2445/191256
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DC Field | Value | Language |
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dc.contributor.author | Cañada García, Javier E. | - |
dc.contributor.author | Moure, Zaira | - |
dc.contributor.author | Sola Campoy, Pedro J. | - |
dc.contributor.author | Delgado Valverde, Mercedes | - |
dc.contributor.author | Cano, María E. | - |
dc.contributor.author | Gijón, Desirèe | - |
dc.contributor.author | González, Mónica | - |
dc.contributor.author | Gracia Ahufinger, Irene | - |
dc.contributor.author | Larrosa, Nieves | - |
dc.contributor.author | Mulet, Xavier | - |
dc.contributor.author | Pitart, Cristina | - |
dc.contributor.author | Rivera, Alba | - |
dc.contributor.author | Bou, Germán | - |
dc.contributor.author | Calvo, Jorge | - |
dc.contributor.author | Cantón, Rafael | - |
dc.contributor.author | González-López, Juan José | - |
dc.contributor.author | Martínez-Martínez, Luis | - |
dc.contributor.author | Navarro, Ferran | - |
dc.contributor.author | Oliver, Antonio | - |
dc.contributor.author | Palacios Baena, Zaira R. | - |
dc.contributor.author | Pascual, Alvaro | - |
dc.contributor.author | Ruiz Carrascoso, Guillermo | - |
dc.contributor.author | Vila Estapé, Jordi | - |
dc.contributor.author | Aracil, Belén | - |
dc.contributor.author | Pérez-Vázquez, María | - |
dc.contributor.author | Oteo Iglesias, Jesús | - |
dc.contributor.author | GEMARA/GEIRAS-SEIMC/REIPI CARB-ES-19 Study Group | - |
dc.date.accessioned | 2022-11-29T16:03:47Z | - |
dc.date.available | 2022-11-29T16:03:47Z | - |
dc.date.issued | 2022-06-30 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/2445/191256 | - |
dc.description.abstract | CARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain. Methods: In total, 71 hospitals, representing all 50 Spanish provinces, collected the first 10 isolates per hospital (February to May 2019); CPE isolates were first identified according to EUCAST (meropenem MIC > 0.12 mg/L with immunochromatography, colorimetric tests, carbapenem inactivation, or carbapenem hydrolysis with MALDI-TOF). Prevalence and incidence were calculated according to population denominators. Antibiotic susceptibility testing was performed using the microdilution method (EUCAST). All 403 isolates collected were sequenced for high-resolution single-nucleotide polymorphism (SNP) typing, core genome multilocus sequence typing (cgMLST), and resistome analysis. Results: In total, 377 (93.5%) CP-Kpn and 26 (6.5%) CP-Eco isolates were collected from 62 (87.3%) hospitals in 46 (92%) provinces. CP-Kpn was more prevalent in the blood (5.8%, 50/853) than in the urine (1.4%, 201/14,464). The cumulative incidence for both CP-Kpn and CP-Eco was 0.05 per 100 admitted patients. The main carbapenemase genes identified in CP-Kpn were bla OXA-48 (263/377), bla KPC-3 (62/377), bla VIM-1 (28/377), and bla NDM-1 (12/377). All isolates were susceptible to at least two antibiotics. Interregional dissemination of eight high-risk CP-Kpn clones was detected, mainly ST307/OXA-48 (16.4%), ST11/OXA-48 (16.4%), and ST512-ST258/KPC (13.8%). ST512/KPC and ST15/OXA-48 were the most frequent bacteremia-causative clones. The average number of acquired resistance genes was higher in CP-Kpn (7.9) than in CP-Eco (5.5). Conclusion: This study serves as a first step toward WGS integration in the surveillance of carbapenemase-producing Enterobacterales in Spain. We detected important epidemiological changes, including increased CP-Kpn and CP-Eco prevalence and incidence compared to previous studies, wide interregional dissemination, and increased dissemination of high-risk clones, such as ST307/OXA-48 and ST512/KPC-3. | - |
dc.format.extent | 13 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2022.918362 | - |
dc.relation.ispartof | Frontiers in Microbiology, 2022, vol. 13, p. 918362 | - |
dc.relation.uri | https://doi.org/10.3389/fmicb.2022.918362 | - |
dc.rights | cc-by (c) Cañada García, Javier E. et al., 2022 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Antibiòtics betalactàmics | - |
dc.subject.classification | Klebsiella pneumoniae | - |
dc.subject.classification | Escheríchia coli | - |
dc.subject.classification | Genoma humà | - |
dc.subject.classification | Enterobacteriàcies | - |
dc.subject.other | Beta lactam antibiotics | - |
dc.subject.other | Klebsiella pneumoniae | - |
dc.subject.other | Escherichia coli | - |
dc.subject.other | Human genome | - |
dc.subject.other | Enterobacteriaceae | - |
dc.title | CARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 725629 | - |
dc.date.updated | 2022-11-29T16:03:47Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 35847090 | - |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) Articles publicats en revistes (ISGlobal) |
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File | Description | Size | Format | |
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725629.pdf | 3.81 MB | Adobe PDF | View/Open |
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