Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/191256
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dc.contributor.authorCañada García, Javier E.-
dc.contributor.authorMoure, Zaira-
dc.contributor.authorSola Campoy, Pedro J.-
dc.contributor.authorDelgado Valverde, Mercedes-
dc.contributor.authorCano, María E.-
dc.contributor.authorGijón, Desirèe-
dc.contributor.authorGonzález, Mónica-
dc.contributor.authorGracia Ahufinger, Irene-
dc.contributor.authorLarrosa, Nieves-
dc.contributor.authorMulet, Xavier-
dc.contributor.authorPitart, Cristina-
dc.contributor.authorRivera, Alba-
dc.contributor.authorBou, Germán-
dc.contributor.authorCalvo, Jorge-
dc.contributor.authorCantón, Rafael-
dc.contributor.authorGonzález-López, Juan José-
dc.contributor.authorMartínez-Martínez, Luis-
dc.contributor.authorNavarro, Ferran-
dc.contributor.authorOliver, Antonio-
dc.contributor.authorPalacios Baena, Zaira R.-
dc.contributor.authorPascual, Alvaro-
dc.contributor.authorRuiz Carrascoso, Guillermo-
dc.contributor.authorVila Estapé, Jordi-
dc.contributor.authorAracil, Belén-
dc.contributor.authorPérez-Vázquez, María-
dc.contributor.authorOteo Iglesias, Jesús-
dc.contributor.authorGEMARA/GEIRAS-SEIMC/REIPI CARB-ES-19 Study Group-
dc.date.accessioned2022-11-29T16:03:47Z-
dc.date.available2022-11-29T16:03:47Z-
dc.date.issued2022-06-30-
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/2445/191256-
dc.description.abstractCARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain. Methods: In total, 71 hospitals, representing all 50 Spanish provinces, collected the first 10 isolates per hospital (February to May 2019); CPE isolates were first identified according to EUCAST (meropenem MIC > 0.12 mg/L with immunochromatography, colorimetric tests, carbapenem inactivation, or carbapenem hydrolysis with MALDI-TOF). Prevalence and incidence were calculated according to population denominators. Antibiotic susceptibility testing was performed using the microdilution method (EUCAST). All 403 isolates collected were sequenced for high-resolution single-nucleotide polymorphism (SNP) typing, core genome multilocus sequence typing (cgMLST), and resistome analysis. Results: In total, 377 (93.5%) CP-Kpn and 26 (6.5%) CP-Eco isolates were collected from 62 (87.3%) hospitals in 46 (92%) provinces. CP-Kpn was more prevalent in the blood (5.8%, 50/853) than in the urine (1.4%, 201/14,464). The cumulative incidence for both CP-Kpn and CP-Eco was 0.05 per 100 admitted patients. The main carbapenemase genes identified in CP-Kpn were bla OXA-48 (263/377), bla KPC-3 (62/377), bla VIM-1 (28/377), and bla NDM-1 (12/377). All isolates were susceptible to at least two antibiotics. Interregional dissemination of eight high-risk CP-Kpn clones was detected, mainly ST307/OXA-48 (16.4%), ST11/OXA-48 (16.4%), and ST512-ST258/KPC (13.8%). ST512/KPC and ST15/OXA-48 were the most frequent bacteremia-causative clones. The average number of acquired resistance genes was higher in CP-Kpn (7.9) than in CP-Eco (5.5). Conclusion: This study serves as a first step toward WGS integration in the surveillance of carbapenemase-producing Enterobacterales in Spain. We detected important epidemiological changes, including increased CP-Kpn and CP-Eco prevalence and incidence compared to previous studies, wide interregional dissemination, and increased dissemination of high-risk clones, such as ST307/OXA-48 and ST512/KPC-3.-
dc.format.extent13 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherFrontiers Media-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.3389/fmicb.2022.918362-
dc.relation.ispartofFrontiers in Microbiology, 2022, vol. 13, p. 918362-
dc.relation.urihttps://doi.org/10.3389/fmicb.2022.918362-
dc.rightscc-by (c) Cañada García, Javier E. et al., 2022-
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.sourceArticles publicats en revistes (Fonaments Clínics)-
dc.subject.classificationAntibiòtics betalactàmics-
dc.subject.classificationKlebsiella pneumoniae-
dc.subject.classificationEscheríchia coli-
dc.subject.classificationGenoma humà-
dc.subject.classificationEnterobacteriàcies-
dc.subject.otherBeta lactam antibiotics-
dc.subject.otherKlebsiella pneumoniae-
dc.subject.otherEscherichia coli-
dc.subject.otherHuman genome-
dc.subject.otherEnterobacteriaceae-
dc.titleCARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3.-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec725629-
dc.date.updated2022-11-29T16:03:47Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.pmid35847090-
Appears in Collections:Articles publicats en revistes (Fonaments Clínics)
Articles publicats en revistes (ISGlobal)

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