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Title: Membrane protein stabilization strategies for structural and functional studies
Author: Errasti-Murugarren, Ekaitz
Bartoccioni, Paola
Palacín Prieto, Manuel
Keywords: Proteïnes de membrana
Membrane proteins
Issue Date: 22-Feb-2021
Publisher: MDPI
Abstract: Accounting for nearly two-thirds of known druggable targets, membrane proteins are highly relevant for cell physiology and pharmacology. In this regard, the structural determination of pharmacologically relevant targets would facilitate the intelligent design of new drugs. The structural biology of membrane proteins is a field experiencing significant growth as a result of the development of new strategies for structure determination. However, membrane protein preparation for structural studies continues to be a limiting step in many cases due to the inherent instability of these molecules in non-native membrane environments. This review describes the approaches that have been developed to improve membrane protein stability. Membrane protein mutagenesis, detergent selection, lipid membrane mimics, antibodies, and ligands are described in this review as approaches to facilitate the production of purified and stable membrane proteins of interest for structural and functional studies.
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It is part of: Membranes, 2021, vol. 11, num. 2
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ISSN: 2077-0375
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

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