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Title: | Reduction of Obesity and Insulin Resistance through Dual Targeting of VAT and BAT by a Novel Combination of Metabolic Cofactors |
Author: | Quesada-Vázquez, Sergio Antolín, Anna Colom-Pellicer, Marina Aragonès, Gerard Herrero Rodríguez, Laura Del Bas, Josep Maria Caimari, Antoni Escoté, Xavier |
Keywords: | Obesitat Resistència a la insulina Teixit adipós Obesity Insulin resistance Adipose tissues |
Issue Date: | 2022 |
Publisher: | MDPI |
Abstract: | Obesity is an epidemic disease worldwide, characterized by excessive fat accumulation associated with several metabolic perturbations, such as metabolic syndrome, insulin resistance, hypertension, and dyslipidemia. To improve this situation, a specific combination of metabolic cofactors (MC) (betaine, N-acetylcysteine, L-carnitine, and nicotinamide riboside) was assessed as a promising treatment in a high-fat diet (HFD) mouse model. Obese animals were distributed into two groups, orally treated with the vehicle (obese + vehicle) or with the combination of metabolic cofactors (obese + MC) for 4 weeks. Body and adipose depots weights; insulin and glucose tolerance tests; indirect calorimetry; and thermography assays were performed at the end of the intervention. Histological analysis of epidydimal white adipose tissue (EWAT) and brown adipose tissue (BAT) was carried out, and the expression of key genes involved in both fat depots was characterized by qPCR. We demonstrated that MC supplementation conferred a moderate reduction of obesity and adiposity, an improvement in serum glucose and lipid metabolic parameters, an important improvement in lipid oxidation, and a decrease in adipocyte hypertrophy. Moreover, MC-treated animals presented increased adipose gene expression in EWAT related to lipolysis and fatty acid oxidation. Furthermore, MC supplementation reduced glucose intolerance and insulin resistance, with an increased expression of the glucose transporter Glut4; and decreased fat accumulation in BAT, raising non-shivering thermogenesis. This treatment based on a specific combination of metabolic cofactors mitigates important pathophysiological characteristics of obesity, representing a promising clinical approach to this metabolic disease. Keywords: obesity; adipose tissue; insulin resistance; thermogenesis; metabolic cofactors |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms232314923 |
It is part of: | International Journal of Molecular Sciences, 2022, vol. 23, p. 14923 |
URI: | http://hdl.handle.net/2445/193229 |
Related resource: | https://doi.org/10.3390/ijms232314923 |
ISSN: | 1661-6596 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) |
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