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Title: | The Multivalency of the glucocorticoid receptor ligand-binding domain explains its manifold physiological activities |
Author: | Jiménez Panizo, Alba Alegre-Martí, Andrea Tettey, Theophilus T. Fettweis, Gregory Abella, Montserrat Antón, Rosa Johnson, Thomas A. Kim, Sohyoung Schiltz, R. Louis Núñez-Barrios, Israel Font-Díaz, Joan Caelles Franch, Carme Valledor Fernández, Annabel Pérez, Paloma Rojas Mendoza, Ana María Fernández-Recio, Juan Presman, Diego M. Hager, Gordon L. Fuentes-Prior, Pablo Estébanez Perpiñá, Eva |
Keywords: | Glucocorticoides Metabolisme Glucocorticoids Metabolism |
Issue Date: | 5-Dec-2022 |
Publisher: | Oxford University Press |
Abstract: | The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that controls metabolic and homeostatic processes essential for life. Although numerous crystal structures of the GR ligand-binding domain (GR-LBD) have been reported, the functional oligomeric state of the full-length receptor, which is essential for its transcriptional activity, remains disputed. Here we present five new crystal structures of agonist-bound GR-LBD, along with a thorough analysis of previous structural work. We identify four distinct homodimerization interfaces on the GR-LBD surface, which can associate into 20 topologically different homodimers. Biologically relevant homodimers were identified by studying a battery of GR point mutants including crosslinking assays in solution, quantitative fluorescence microscopy in living cells, and transcriptomic analyses. Our results highlight the relevance of non-canonical dimerization modes for GR, especially of contacts made by loop L1-3 residues such as Tyr545. Our work illustrates the unique flexibility of GR's LBD and suggests different dimeric conformations within cells. In addition, we unveil pathophysiologically relevant quaternary assemblies of the receptor with important implications for glucocorticoid action and drug design. |
Note: | Reproducció del document publicat a: https://doi.org/10.1093/nar/gkac1119 |
It is part of: | Nucleic Acids Research, 2022, vol. 30, num. 22, p. 13063-13082 |
URI: | http://hdl.handle.net/2445/193850 |
Related resource: | https://doi.org/10.1093/nar/gkac1119 |
ISSN: | 0305-1048 |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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