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Title: | Distinct DNA methylomes of newborns and centenarians |
Author: | Heyn, Holger Li, Ning Ferreira, Humberto J. Moran, Sebastian Pisano, David G. Gomez, Antonio Diez, Javier Sanchez-Mut, Jose Vicente Setien, Fernando Javier Carmona, F. Puca, Annibale A. Sayols, Sergi Pujana, Miguel A. Serra-Musach, Jordi Iglesias Platas, Isabel Formiga Pérez, Francesc Fernandez, Agustin F. Fraga, Mario F. Heath, Simon C. Valencia, Alfonso Gut, Ivo G. Wang, Jun Esteller, Manel |
Keywords: | Envelliment ADN Infants nadons Aging DNA Newborn infants |
Issue Date: | 26-Jun-2012 |
Publisher: | National Academy of Sciences |
Abstract: | Human aging cannot be fully understood in terms of the constrained genetic setting. Epigenetic drift is an alternative means of explaining age-associated alterations. To address this issue, we performed whole-genome bisulfite sequencing (WGBS) of newborn and centenarian genomes. The centenarian DNA had a lower DNA methylation content and a reduced correlation in the methylation status of neighboring cytosine--phosphate--guanine (CpGs) throughout the genome in comparison with the more homogeneously methylated newborn DNA. The more hypomethylated CpGs observed in the centenarian DNA compared with the neonate covered all genomic compartments, such as promoters, exonic, intronic, and intergenic regions. For regulatory regions, the most hypomethylated sequences in the centenarian DNA were present mainly at CpG-poor promoters and in tissue-specific genes, whereas a greater level of DNA methylation was observed in CpG island promoters. We extended the study to a larger cohort of newborn and nonagenarian samples using a 450,000 CpG-site DNA methylation microarray that reinforced the observation of more hypomethylated DNA sequences in the advanced age group. WGBS and 450,000 analyses of middle-age individuals demonstrated DNA methylomes in the crossroad between the newborn and the nonagenarian/centenarian groups. Our study constitutes a unique DNA methylation analysis of the extreme points of human life at a single-nucleotide resolution level. |
Note: | Reproducció del document publicat a: https://doi.org/10.1073/pnas.1120658109 |
It is part of: | Proceedings of the National Academy of Sciences of the United States of America - PNAS, 2012, vol. 109, num. 26, p. 10522-10527 |
URI: | http://hdl.handle.net/2445/193857 |
Related resource: | https://doi.org/10.1073/pnas.1120658109 |
ISSN: | 0027-8424 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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