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Title: | High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization |
Author: | Wu, Vincen Tillner, Jocelyn Jones, Emrys McKenzie, James S Gurung, Dipa Mroz, Anna Poynter, Liam Simon, Daniel Grau, Cristina Altafaj, Xavier Dumas, Marc-Emmanuel Gilmore, Ian Bunch, Josephine Takats, Zoltan |
Keywords: | Cervell Diagnòstic per la imatge Espectrometria de masses Espectrometria de masses amb ionització per electroesprai Ratolins (Animals de laboratori) Brain Diagnostic imaging Mass spectrometry Electrospray ionization mass spectrometry Mice (Laboratory animals) |
Issue Date: | 19-Jul-2022 |
Publisher: | American Chemical Society |
Abstract: | In this study, we examine the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue. We also compare the performance of desorption electrospray ionization (DESI) with and without the flow focusing setup. The main potential advantages of applying the flow focusing mechanism in DESI is its rotationally symmetric electrospray jet, higher intensity, more controllable parameters, and better portability due to the robustness of the sprayer. The parameters for DEFFI have therefore been thoroughly optimized, primarily for spatial resolution but also for intensity. Once the parameters have been optimized, DEFFI produces similar images to the existing DESI. MS images for mouse brain samples, acquired at a nominal pixel size of 50 μm, are comparable for both DESI setups, albeit the new sprayer design yields better sensitivity. Furthermore, the two methods are compared with regard to spectral intensity as well as the area of the desorbed crater on rhodamine-coated slides. Overall, the implementation of a flow focusing mechanism in DESI is shown to be highly suitable for imaging biological tissue and has potential to overcome some of the shortcomings experienced with the current geometrical design of DESI. |
Note: | Reproducció del document publicat a: https://doi.org/10.1021/acs.analchem.2c00345 |
It is part of: | Analytical Chemistry, 2022, vol. 94, num. 28, p. 10035-10044 |
URI: | http://hdl.handle.net/2445/194393 |
Related resource: | https://doi.org/10.1021/acs.analchem.2c00345 |
ISSN: | 0003-2700 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
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