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http://hdl.handle.net/2445/194492
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DC Field | Value | Language |
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dc.contributor.author | Akkermans, Onno | - |
dc.contributor.author | Delloye-Bourgeois, Céline | - |
dc.contributor.author | Peregrina, Claudia | - |
dc.contributor.author | Carrasquero Ordaz, Maria | - |
dc.contributor.author | Kokolaki, Maria | - |
dc.contributor.author | Berbeira Santana, Miguel | - |
dc.contributor.author | Chavent, Matthieu | - |
dc.contributor.author | Reynaud, Florie | - |
dc.contributor.author | Raj, Ritu | - |
dc.contributor.author | Agirre, Jon | - |
dc.contributor.author | Aksu, Metin | - |
dc.contributor.author | S. White, Eleanor | - |
dc.contributor.author | Lowe, Edward | - |
dc.contributor.author | Ben Amar, Dounia | - |
dc.contributor.author | Zaballa, Sofía | - |
dc.contributor.author | Huo, Jiandong | - |
dc.contributor.author | Pakos, Irene | - |
dc.contributor.author | McCubbin, Patrick | - |
dc.contributor.author | Comoletti, Davide | - |
dc.contributor.author | Owens, Raymond J. | - |
dc.contributor.author | Robinson, Carol V. | - |
dc.contributor.author | Castellani, Valérie | - |
dc.contributor.author | Del Toro, Daniel | - |
dc.contributor.author | Seiradake, Elena | - |
dc.date.accessioned | 2023-03-02T18:07:20Z | - |
dc.date.available | 2023-10-13T05:10:32Z | - |
dc.date.issued | 2022-10-13 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | http://hdl.handle.net/2445/194492 | - |
dc.description.abstract | Neural migration is a critical step during brain development that requires the interactions of cell-surface guidance receptors. Cancer cells often hijack these mechanisms to disseminate. Here, we reveal crystal structures of Uncoordinated-5 receptor D (Unc5D) in complex with morphogen receptor glypican-3 (GPC3), forming an octameric glycoprotein complex. In the complex, four Unc5D molecules pack into an antiparallel bundle, flanked by four GPC3 molecules. Central glycan-glycan interactions are formed by N-linked glycans emanating from GPC3 (N241 in human) and C-mannosylated tryptophans of the Unc5D thrombospondin-like domains. MD simulations, mass spectrometry and structure-based mutants validate the crystallographic data. Anti-GPC3 nanobodies enhance or weaken Unc5-GPC3 binding and, together with mutant proteins, show that Unc5/GPC3 guide migrating pyramidal neurons in the mouse cortex, and cancer cells in an embryonic xenograft neuroblastoma model. The results demonstrate a conserved structural mechanism of cell guidance, where finely balanced Unc5-GPC3 interactions regulate cell migration. | - |
dc.format.extent | 46 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Cell Press | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.cell.2022.09.025 | - |
dc.relation.ispartof | Cell, 2022, vol. 185, num. 1, p. 3931-3949 | - |
dc.relation.uri | https://doi.org/10.1016/j.cell.2022.09.025 | - |
dc.rights | cc-by-nc-nd (c) Akkermans, Onno et al., 2022 | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Migració cel·lular | - |
dc.subject.classification | Escorça cerebral | - |
dc.subject.classification | Cristal·lografia | - |
dc.subject.classification | Ressonància de plasmons superficials | - |
dc.subject.other | Cell migration | - |
dc.subject.other | Cerebral cortex | - |
dc.subject.other | Crystallography | - |
dc.subject.other | Surface plasmon resonance | - |
dc.title | GPC3-Unc5 receptor complex structure and role in cell migration | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 731945 | - |
dc.date.updated | 2023-03-02T18:07:21Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 36240740 | - |
Appears in Collections: | Articles publicats en revistes (Institut de Neurociències (UBNeuro)) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) |
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731945.pdf | 16.35 MB | Adobe PDF | View/Open |
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