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DC Field | Value | Language |
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dc.contributor.author | Perpiñán, Elena | - |
dc.contributor.author | Pérez-Del-Pulgar, Sofía | - |
dc.contributor.author | Londoño, María Carlota | - |
dc.contributor.author | Mariño, Zoe | - |
dc.contributor.author | Bartrés, Concepció | - |
dc.contributor.author | González, Patricia | - |
dc.contributor.author | García-López, Mireia | - |
dc.contributor.author | Pose, Elisa | - |
dc.contributor.author | Lens, Sabela | - |
dc.contributor.author | Maini, Mala K | - |
dc.contributor.author | Forns, Xavier | - |
dc.contributor.author | Koutsoudakis, George | - |
dc.date.accessioned | 2023-03-14T14:12:35Z | - |
dc.date.available | 2023-03-14T14:12:35Z | - |
dc.date.issued | 2020-02-25 | - |
dc.identifier.issn | 1664-3224 | - |
dc.identifier.uri | http://hdl.handle.net/2445/195230 | - |
dc.description.abstract | Background: Chronic hepatitis C virus (HCV) infection impairs natural killer (NK) cell phenotype and function. Whether restoration of NK cells occurs after successful interferon (IFN)-free therapies remains a controversial issue. Aim: To analyze how HCV-related liver cirrhosis impacts changes in NK cells prior and post-IFN-free therapies. Methods: NK cell analysis by multicolor flow cytometry was performed in HCV-infected patients with (n = 17) and without (n = 14) cirrhosis at baseline, week 4 during therapy, and weeks 12 and 48 after the end of therapy (FU12 and FU48, respectively). Non-HCV cirrhotic patients (n = 12) and healthy individuals (n = 12) served as controls. Results: At baseline, HCV cirrhotic patients presented an altered distribution of NK subsets (CD56dim and CD56bright) with higher expression of NKp46, HLA-DR, NKp30, KIR2DL2/L3, NKG2A, and CD85j receptors compared to healthy controls. All frequencies normalized by FU48, except for CD85j+ cells. Likewise, substantial alterations were detected in NK cell function assessed by (i) signal transducer and activator of transcription 1 (STAT1) and phosphorylated levels of STAT1 and STAT4, (ii) degranulation (CD107a), (iii) cytotoxicity [tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)], and (iv) cytokine production [IFN-γ and tumor necrosis factor-α (TNF-α)]. Of note, NK cell function at FU48 remained partially impaired. In contrast, non-cirrhotics showed normal baseline frequencies of HLA-DR-, NKG2A-, and CD85j-expressing NK cells. Importantly, altered baseline frequencies of NK cell subsets and NKp46+ CD56dim cells, as well as NK cell function, were rapidly and completely restored. Conclusions: NK cell phenotype alterations persist after HCV eradication in cirrhotic patients, while their function is only partially restored, compromising immune restoration and immunosurveillance | - |
dc.format.extent | 15 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fimmu.2020.00129 | - |
dc.relation.ispartof | Frontiers in Immunology, 2020, vol. 11, p. 129 | - |
dc.relation.uri | https://doi.org/10.3389/fimmu.2020.00129 | - |
dc.rights | cc-by (c) Perpiñán, Elena et al., 2020 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Articles publicats en revistes (Medicina) | - |
dc.subject.classification | Virus de l'hepatitis C | - |
dc.subject.classification | Cirrosi hepàtica | - |
dc.subject.classification | Interferó | - |
dc.subject.classification | Citometria de fluxe | - |
dc.subject.other | Hepatitis C virus | - |
dc.subject.other | Hepatic cirrhosis | - |
dc.subject.other | Interferon | - |
dc.subject.other | Flow cytometry | - |
dc.title | Cirrhosis Hampers Early and Rapid Normalization of Natural Killer Cell Phenotype and Function in Hepatitis C Patients Undergoing Interferon-Free Therapy | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 712017 | - |
dc.date.updated | 2023-03-14T14:12:35Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 32161581 | - |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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712017.pdf | 7.71 MB | Adobe PDF | View/Open |
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