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DC Field | Value | Language |
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dc.contributor.author | Bonacorsi, Stéphane | - |
dc.contributor.author | Visseaux, Benoit | - |
dc.contributor.author | Bouzid, Donia | - |
dc.contributor.author | Pareja, Josep | - |
dc.contributor.author | Rao, Sonia N. | - |
dc.contributor.author | Manissero, Davide | - |
dc.contributor.author | Hansen, Glen | - |
dc.contributor.author | Vila Estapé, Jordi | - |
dc.date.accessioned | 2023-03-15T14:37:44Z | - |
dc.date.available | 2023-03-15T14:37:44Z | - |
dc.date.issued | 2021-09-23 | - |
dc.identifier.issn | 2296-858X | - |
dc.identifier.uri | http://hdl.handle.net/2445/195311 | - |
dc.description.abstract | Background: Quantitative (q) polymerase chain reaction (PCR) cycle threshold (Ct) values represent the number of amplification cycles required for a positive PCR result and are a proxy of pathogen quantity in the tested sample. The clinical utility of Ct values remains unclear for gastrointestinal infections. Objectives: This systematic review assesses the global medical literature for associations between Ct values of gastrointestinal pathogens and patient presentation and clinical outcomes. Data Sources: MEDLINE, EMBASE, Cochrane library databases: searched January 14-17, 2020. Study Eligibility Criteria: Studies reporting on the presence or absence of an association between Ct values and clinical outcomes in adult and pediatric populations were included. Animal studies, reviews, meta-analyses, and non-English language studies were excluded. Participants: Humans infected with gastrointestinal pathogens, detected with qPCR. Interventions: Diagnostics assessing Ct values. Extracted data were reported narratively. Results: Thirty-three eligible studies were identified; the most commonly studied pathogens were Clostridioides difficile (n = 15), norovirus (n = 10), and rotavirus (n = 9). Statistically significant associations between low C. difficile Ct values and increased symptom severity or poor outcome were reported in 4/8 (50%) studies, and increased risk of death in 1/2 (50%) studies; no significant associations were found between Ct value and duration of symptoms or length of hospital stay. Among studies of norovirus, 5/7 (71%), mainly genogroup II, reported symptomatic cases with significantly lower median Ct values than controls. Significantly lower rotavirus Ct values were also observed in symptomatic cases vs. controls in 3/7 (43%) studies, and associated with more severe symptoms in 2/2 studies. Contradictory associations were identified for non-C. difficile bacterial and parasitic pathogens. Conclusions: In conclusion, some studies reported clinically useful associations between Ct values and patient or healthcare outcomes; additional, well-designed, large-scale trials are warranted based on these findings. Systematic Review Registration: [PROSPERO], identifier [CRD42020167239]. | - |
dc.format.extent | 18 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fmed.2021.711809 | - |
dc.relation.ispartof | Frontiers in Medicine, 2021, vol. 8, p. 711809 | - |
dc.relation.uri | https://doi.org/10.3389/fmed.2021.711809 | - |
dc.rights | cc-by (c) Bonacorsi, Stéphane et al., 2021 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Articles publicats en revistes (Fonaments Clínics) | - |
dc.subject.classification | Reacció en cadena de la polimerasa | - |
dc.subject.classification | Malalties del tracte gastrointestinal | - |
dc.subject.classification | Microbiota intestinal | - |
dc.subject.classification | Bacteris patògens | - |
dc.subject.classification | Ressenyes sistemàtiques (Investigació mèdica) | - |
dc.subject.other | Polymerase chain reaction | - |
dc.subject.other | Gastrointestinal system diseases | - |
dc.subject.other | Gastrointestinal microbiome | - |
dc.subject.other | Pathogenic bacteria | - |
dc.subject.other | Systematic reviews (Medical research) | - |
dc.title | Review on the Correlation of Quantitative PCR Cycle Threshold Values of Gastrointestinal Pathogens with Patient Clinical Presentation and Outcomes | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 716000 | - |
dc.date.updated | 2023-03-15T14:37:44Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 34631732 | - |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) Articles publicats en revistes (ISGlobal) |
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